Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Detection kit for insulin-like growth factor binding protein-1

A detection kit and insulin-like technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of poor accuracy, sensitivity, repeatability, unsuitable for clinical use, cumbersome operation, etc., and achieve little hemolysis interference , high specificity, quick and easy operation

Active Publication Date: 2015-03-25
ZYBIO INC
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Currently known IGFBP-1 detection methods still have many defects, such as cumbersome operation, long time-consuming, not suitable for clinical use
The accuracy, sensitivity and repeatability of IGFBP-1 detection kits on the Chinese market are still poor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection kit for insulin-like growth factor binding protein-1
  • Detection kit for insulin-like growth factor binding protein-1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Preparation of test paper card of the present invention:

[0047] 1) Use the pretreatment buffer to pretreat the gold standard pad. The pretreatment buffer is: stachyose 2g / L, alum 0.5g / L, fructose sodium diphosphate 1.5g / L, sodium hexametaphosphate 0.3g / L L, glycine 1.88g / L aqueous solution, pH = 7.4, the specific steps of pretreatment are: soak the gold standard pad in the pretreatment solution for 2 hours, take it out and dry it at 37°C; then use fluorescent microspheres to label the insulin sample The growth factor binding protein-1 antibody solution was sprayed on the pretreated gold label pad to prepare the gold label pad coated with the insulin-like growth factor binding protein-1 antibody, and the mass ratio of the fluorescent microspheres to the antibody in the solution was 5:1. The concentration of the solution is 10mg / ml, and the spray volume is 4ul / cm;

[0048] 2) Spray 1mg / ml insulin-like growth factor binding protein-1 antibody solution and goat anti-mous...

Embodiment 2

[0062] Preparation of comparative test paper card:

[0063] The gold standard pad was pretreated with 25mM glycine buffer solution, and other reagents and experimental methods were the same as in Example 1.

[0064] 1) Pretreat the gold label pad with 25mM glycine buffer solution, and then spray the pretreated gold label pad with fluorescent microsphere-labeled insulin-like growth factor binding protein-1 antibody solution to prepare the coated insulin-like growth factor binding protein-1 antibody solution. 1 Antibody gold label pad, the mass ratio of fluorescent microspheres to antibody in the solution is 5:1, the concentration of the solution is 10mg / ml, and the spraying amount is 4ul / cm;

[0065] 2) Spray 1mg / ml insulin-like growth factor binding protein-1 antibody solution and goat anti-mouse antibody solution on the test line and quality control line of the nitrocellulose membrane respectively, the spraying amount is 1ul / cm, and the coated nitrocellulose membrane;

[00...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention relates to the field of bio-detection and particularly relates to a detection kit for quantitatively detecting an insulin-like growth factor binding protein-1, a preparation method and use thereof. The detection kit for the insulin-like growth factor binding protein-1 provided by the invention comprises a test paper card which comprises a bottom plate as well as a sample pad, a gold label pad, a nitrocellulose membrane and a water absorption pad which are sequentially arranged from a sampling end on the surface of the bottom plate; the gold label pad comprises an insulin-like growth factor binding protein-1 antibody, a detection line and a quality control line are coated on the nitrocellulose membrane, and the insulin-like growth factor binding protein-1 antibody on the gold label pad is labeled by using a fluorescent microsphere. The kit provided by the invention can be used for detecting the insulin-like growth factor binding protein-1 by virtue of a fluorescent microsphere immunochromatography technology for the first time, has sensitivity and specificity and has the advantages of fast, simple and convenient operation, accurate result, economical benefit and practicality and the like.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to an insulin-like growth factor binding protein-1 detection kit and its preparation method and application. Background technique [0002] Insulin-like growth factor binding protein-1 (IGFBP-1) is the best method for detecting rupture of membranes, and it is also the gold standard approved by the US FDA. There are different titles such as premature rupture of membranes test strips, hyperphosphorylation, hypophosphorylation and non-phosphorylation clinically in various countries in the world, all of which refer to the detection method of insulin-like growth factor binding protein-1. This method has been clinically proven to be superior to other similar detection methods. The dark blue IGF-binding protein-1 detection strip has high accuracy (greater than 99%) and is easy to use. Whether in obstetrics and gynecology clinics, hospital examination rooms, outpatient clinics or at home...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/533G01N33/68
CPCG01N33/533G01N33/577G01N33/68
Inventor 吉权
Owner ZYBIO INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com