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Application of thermostable carbonic anhydrase in catalyzing the cyclic absorption-desorption of CO2 in organic amine solution

A technology of carbonic anhydrase and thermal stability, which is applied in lyase, chemical instruments and methods, biosynthesis, etc., can solve the problem of not many applications of CA, and achieve the effect of stable catalytic ability, good stability, and good stability

Active Publication Date: 2016-03-23
LIVINGZONE SHANGHAI BIO CHEM TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many studies at home and abroad have proved that CA can effectively promote the absorption of CO by organic solvents. 2 efficiency, but the application of CA in desorption is still not much

Method used

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  • Application of thermostable carbonic anhydrase in catalyzing the cyclic absorption-desorption of CO2 in organic amine solution
  • Application of thermostable carbonic anhydrase in catalyzing the cyclic absorption-desorption of CO2 in organic amine solution
  • Application of thermostable carbonic anhydrase in catalyzing the cyclic absorption-desorption of CO2 in organic amine solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The preparation of embodiment 1PaCA enzyme liquid

[0044] 1.1 Medium:

[0045] LB solid medium (g / L): yeast extract 5, peptone 10, sodium chloride 10, agar 15

[0046] LB liquid medium (g / L): yeast extract 5, peptone 10, sodium chloride 10

[0047] 1.2 Preparation steps

[0048] The carbonic anhydrase nucleotide sequence (SeqNo.1, named PaCA) was obtained according to the Blast comparison. After optimization, the whole gene was synthesized by Nanjing GenScript Biotechnology Co., Ltd., and then ligated with the plasmid pET21a (Novagen) for recombination Plasmid PaCA-pET21a was transformed into BL21(DE3) competent cells (purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd.), and a single colony of positive clones was picked for expression verification of soluble active expression.

[0049] Streak the Escherichia coli in the glycerol tube on the plate with ampicillin-resistant LB solid medium. Cultivate at 37°C for 12-16h, pick a single colony and transfe...

Embodiment 2

[0051] Example 2: Thermostable carbonic anhydrase catalyzes organic amine solution cycle absorption-desorption CO 2 Application in: Materials and instruments: The reactor is a 5L jacketed glass reactor (height-to-diameter ratio: 8:1), provided by Shanghai Kexing Glass Instrument Factory.

[0052] N-methyldiethanolamine (MDEA), analytically pure; carbonic anhydrase, self-made (PaCA); CO 2 Gas was provided by Shanghai Xiqin Gas Co., Ltd.

[0053] In this example, the method of cyclic absorption and desorption is used to evaluate the absorption and desorption of CO by PaCA catalyzed organic amine solution. 2 efficiency and enzyme stability.

[0054] The experimental plan is absorption at room temperature - desorption at 80°C (adding 800U / mLPaCA enzyme solution). The absorption system is 2L3MMDEA solution, and CO is fed into the system at a rate of 6L / min. 2 , check the pH every 10 minutes, and weigh the absorption system when the pH value is stable and no longer changes. Aft...

Embodiment 3

[0057] Example 3: Thermostable carbonic anhydrase catalyzes organic amine solution cycle absorption-desorption CO 2 Apps in:

[0058] Material: Monoethanolamine (MEA), analytically pure. Other experimental conditions are the same as in Example 2.

[0059] The experimental plan is absorption at 30°C—desorption at 60°C, adding 900U / mL PaCA enzyme solution, the absorption system is 2L3MMEA solution, and CO is introduced into the system at a rate of 6L / min. 2 , check the pH every 10 minutes, and weigh the absorption system when the pH value is stable and no longer changes. After the absorption, raise the temperature of the reactor to 80°C and start to desorb CO 2 , the stirring rate was 300rpm, the pH change was detected every 5min (pH was measured at 27-28°C), and the desorption time was 60min. The reaction system was weighed and the volume was measured before desorption, and the volume was measured again after desorption. The reduced part was considered to be caused by water...

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Abstract

The invention provides application of heat-stability carbonic anhydrase to circular absorption-desorption of CO2 by catalyzing an organic amine solution, and belongs to the field of CO2 emission reduction treatment. The heat-stability carbonic anhydrase has an amino acid sequence of SEQ ID NO:2 shown in specification. The CO2 can be circularly absorbed and desorbed by organic amine by using carbonic anhydrase (PaCA) obtained by genetic recombination expression; carbonic anhydrase has the relatively stable catalyzing capability in the process of circularly absorbing and desorbing the CO2 by the organic amine; and the relatively good stability still can be kept after the carbonic anhydrase is circulated for multiple times in the application, so that the energy investment can be well saved.

Description

technical field [0001] The invention belongs to CO 2 The field of emission reduction treatment, specifically relates to a thermostable carbonic anhydrase in catalyzing organic amine solution cycle absorption-desorption CO 2 in the application. Background technique [0002] CO 2 It is the main greenhouse gas that causes global warming. In the past 100 years, the large-scale industrialization process of human beings has led to the uncontrolled emission of greenhouse gases into the atmosphere. With the reduction of forest resources, CO 2 Levels have risen beyond the absorption capacity of plants and oceans. There are about 24 billion tons of CO produced globally each year 2 emissions, and nearly 40% of emissions come from power plants around the world. According to statistics, China currently has more than 3,000 coal-fired power plants (about 5,000 in the world). Taking a 300-megawatt power plant as an example, its power generation efficiency is generally around 35%, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/88B01D53/86B01D53/62B01D53/96
CPCY02P20/59
Inventor 祝俊余允东周晓青张燕汪浩龙辉张敏陈亭亭陈风义
Owner LIVINGZONE SHANGHAI BIO CHEM TECH CO LTD
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