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Novel indica-japonica hybrid gene introgression sheath blight resistance breeding method

A technology of gene introgression and sheath blight, applied in the field of agricultural science, can solve the problems of indica and japonica crossing, difficult for breeders to master, and high technical threshold, so as to reduce screening workload, shorten breeding cycle, and low operating threshold Effect

Inactive Publication Date: 2015-03-25
兰进好
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the transgenic breeding of the sheath blight resistance gene and other superior genes for specific elite main cultivars could have accurately achieved the improvement of indica and japonica crosses. , which also requires multi-generation hybrid backcrossing in the field, which cannot solve the problem of indica and japonica crossing. In addition, molecular technology breeding has the problems of high cost and high technical threshold, and it is difficult for ordinary breeders to master

Method used

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  • Novel indica-japonica hybrid gene introgression sheath blight resistance breeding method
  • Novel indica-japonica hybrid gene introgression sheath blight resistance breeding method
  • Novel indica-japonica hybrid gene introgression sheath blight resistance breeding method

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Embodiment

[0049] (1) Screening W09557 and Teqing respectively as japonica rice breeding parents and indica rice sheath blight resistance sources:

[0050] Years of breeding practice have found that the main breeding line W0955 has the characteristics of high yield and relatively good rice quality. At the same time, the average plant height of W0955 measured in two years is only 92.8cm, and the whole growth period is only 146 days. It is susceptible to sheath blight. In 2011 The anthers of W0955 were inoculated in the indica rice medium for induction culture in the positive season. The experiment found that the callus induction rate of W0955 was as high as 48% (the anther culture procedure is the same as before), which is very suitable for the requirements of the japonica rice breeding parent of this technical route. Teqing is highly resistant to multiple sheath blight strains in many areas of sheath blight disease, and is one of the important antigens for sheath blight resistance breedin...

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Abstract

The invention discloses a novel indica-japonica hybrid gene introgression sheath blight resistance breeding method. The novel indica-japonica hybrid gene introgression sheath blight resistance breeding method includes the following steps that (1), an indica-japonica variety / system to be improved and a sheath blight resistance indica-japonica resistance source are selected; (2), the toxin tolerance concentration of the selected indica-japonica variety / system to be improved is roughly identified; (3), a three-way cross F1 hybrid is acquired through cross breeding; (4), row indica culture medium anther culture is conducted on the three-way cross F1 hybrid; (5), anther-cultured seedlings are planted in a large field, multiple indica-japonica anther culture families with the improved sheath blight resistance are acquired through single-seedling harvesting and planting, the different anther culture families are further sown in the large field, a parent of the indica-japonica variety / system to be improved serves as a contrast, and the new indica-japonica strain with the good agronomic character and the improved sheath blight resistance is screened out. By the adoption of the novel indica-japonica hybrid gene introgression sheath blight resistance breeding method, indica-japonica sheath blight resistance genes can specially and efficiently seep into indica-japonica, sheath blight resistance breeding can be finished about three years, a conventional indica-japonica hybrid sheath blight resistance breeding period is remarkably shortened, and the high breeding application value is achieved.

Description

technical field [0001] The invention relates to the field of agricultural science and technology, in particular to a rice breeding method for specifically and efficiently infiltrating an indica rice sheath blight resistance gene into japonica rice, specifically a new indica-japonica cross gene introgression resistance to sheath blight breeding method. Background technique [0002] Rice sheath blight (rice sheath blight), commonly known as rotten feet, flower stalks, and foot blight, is one of the important diseases in rice production and is a disease of rice all over the world. The disease was first discovered in Japan in 1905, and was reported in my country in 1934, and then spread rapidly and spread to Africa, Europe and the Americas. At present, the disease has generally occurred in rice-growing countries in Asia, Africa, Europe, and the United States. It generally reduces production by 10% to 30%, and can reach 50% in severe cases. It poses a great threat to rice produc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H4/00
Inventor 不公告发明人
Owner 兰进好
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