Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular genetics identification method for channel catfishes and flathead catfishes

A technology for channel catfish and channel catfish, which is applied in the field of genetics, can solve the problems of unguaranteed quality, mixing of channel catfish and channel catfish, and no literature on the repeating regularity of microsatellites, and achieves good stability and repeatability. high rate effect

Active Publication Date: 2015-03-18
FRESHWATER FISHERIES RES INSITUTE OF JIANGSUPROVINCE +1
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the germplasm of channel catfish and longpin catfish depends on imports, and the quality cannot be guaranteed. There may be a mixture of channel catfish and longpin catfish
[0003] At present, there is no literature reporting the microsatellite duplication regularity of the channel catfish and long-fin catfish MSTN gene, and this is used as the basis for the molecular identification of the two fishes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular genetics identification method for channel catfishes and flathead catfishes
  • Molecular genetics identification method for channel catfishes and flathead catfishes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Primer design

[0023] Design a pair of specific PCR amplification primers, the primer sequence is: the upstream primer SEQ ID NO. 1: 5'-ACGGTGTTC CTGTTACTGC-3', the downstream primer SEQ ID NO. 2: 5'-CACCAGATGTTGCTATGC-3'.

[0024] 2. Sample collection

[0025] Take 30 channel catfish and longfin catfish to be identified.

[0026] 3. Genomic DNA extraction

[0027] Take about 20mg of the caudal fin tissue of each fish, absorb the surface water with filter paper, put it into a 1.5ml centrifuge tube, add 420μL STE buffer (30mmol / L Tris-HCl, pH8.0, 200mmol / L EDTA, 50mmol / L NaCl) . After mixing, add 80μL of 10% SDS and 10μL of 20mg / mL proteinase K, digest at 56℃ for 8-10h; add equal volume of phenol:chloroform:isoamyl alcohol (25:24:1) for extraction twice, and centrifuge at 12000r 10min, take the supernatant; add an equal volume of chloroform: isoamyl alcohol (24:1) to extract once, centrifuge at 12000r for 10min, take the supernatant; add an equal volume of pre-cooled absolu...

Embodiment 2

[0034] According to the method of Example 1, the sample was expanded to 100 individual channel catfish and longfin catfish. The results still showed that the channel catfish (AGC) microsatellite repeats from the 83rd position (MSTN-83) was six times. The number of repetitions of the (AGC) microsatellite of the catfish from the 83rd position (MSTN-83) is seven.

[0035]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the genetics field and relates to a molecular genetics identification method for channel catfishes and flathead catfishes. The molecular genetics identification method for channel catfishes and flathead catfishes comprises the following steps: respectively extracting genome DNA (deoxyribonucleic acid) of to-be-identified channel catfishes and flathead catfishes; performing PCR (polymerase chain reaction) amplification on amicine genes of the channel catfishes and flathead catfishes; sequencing and comparing; if AGC microsatellites, starting from the 83th locus, of the MSTN (myostatin) gene are repeated for six times, identifying the catfishes as channel catfishes, and if the microsatellites are repeated for seven times, judging the catfishes as flathead catfishes. The molecular genetics identification method disclosed by the invention can be used for quickly, simply and conveniently, accurately and effectively identifying the channel catfishes and the flathead catfishes, is good in result stability and high in repetitive rate, so that the blank of identifying channel catfishes and flathead catfishes according to the existing domestic molecular biologic criteria is filled up.

Description

Technical field [0001] This invention belongs to the field of genetics, and relates to a method for molecular genetic identification of channel catfish and longfin catfish. Background technique [0002] Channel catfish and longfin catfish are native to the United States and are currently the most mature species in the United States with the most mature breeding technology and the highest breeding output, with a breeding output of about 300,000 tons. After the channel catfish was introduced to my country in the 1980s, it has developed rapidly in just over two decades. It has successively broken through the technical difficulties in breeding, breeding, feed, processing and exporting, and has formed a development from seed breeding and breeding. The production of a complete industrial chain of, processing and marketing has also increased sharply from the initial several thousand tons, reaching 224,000 tons at its peak, becoming one of the main products exported to the United States b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 钟立强陈校辉边文冀王明华秦钦陈友明张明生史阳白孔杰栾生
Owner FRESHWATER FISHERIES RES INSITUTE OF JIANGSUPROVINCE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com