A kind of artificial bear bile powder and preparation method thereof

Abstract

The invention discloses an artificial bear bile powder and a preparation method thereof. The preparation method comprises the following steps: a) dissolving poultry bile powder with a buffer solution of pH=7-9 and then filtering to obtain filter residue and filtrate; b ) adding the filtrate to a reactor equipped with 7α-hydroxysteroid dehydrogenase, 7β-hydroxysteroid dehydrogenase and coenzyme I or coenzyme II for reaction; c) freeze-drying the reaction solution after the reaction and combining with the The filter residue is mixed to obtain artificial bear bile powder. The present invention completely uses natural sources of poultry bile as the raw material for artificial bear bile, without adding other chemical substances, and adopts common animal bile, and its bile source is easy to collect, obtain, and process; through in vitro simulation of the enterohepatic circulation process , to bioconvert poultry bile into artificial bear bile powder that is chemically equivalent to natural bear bile powder, as a substitute for natural bear bile, to protect the endangered Asian black bear, and to promote the healthy development of the bear bile industry.

Description

technical field [0001] The invention belongs to the field of medicines, in particular to an artificial bear bile powder and a preparation method thereof. Background technique [0002] Bear bile, obtained from the dried gallbladder bile of black bears or brown bears, has the effects of clearing away heat, calming the liver, and improving eyesight. Its main component is tauroursodeoxycholic acid (TUDCA). Bear bile is one of the four famous and precious animal medicines. It has a history of more than 2,000 years of use, and a large number of prescriptions contain bear bile. my country's annual output of bear bile powder is about 30 tons, which still cannot meet the needs of human health. At present, the contradiction between supply and demand of high-end bear bile powder is increasingly prominent and the market demand for bear bile powder for low-end raw materials continues to increase, which makes the price of bear bile powder rise steadily, and the market price has exceeded ...

AI Technical Summary

Problems solved by technology

[0012] There are still problems with the artificial bear bile prepared by the current technology. In some technologies, the content of tauroursodeoxycholic acid in products made by simply mixing different poultry bile cannot reach the standard of the Pharmacopoeia at all.

Many technologies add some chemical substances such as tauroursodeoxycholic acid, bile pigment, amino acid, etc. to the mixture of poultry bile so that the chemical composition of the finished product is similar to that of natural bear bile powder, but it cannot be truly Become a bear bile substitute

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