Method for detecting potassium salt in cow milk

A milk and potassium salt technology, which is applied in material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem of long detection time, high requirements for instruments and reagents, and cost of milk products. Advanced problems, to achieve the effect of simple operation, low cost, and clear judgment results

Inactive Publication Date: 2015-02-25
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing detection methods for potassium salts in dairy products first need to digest the samples, such as wet digestion, microwave digestion and dry ashing, and then use inductively coupled plasma-atomic emission spectrometry (ICP-AES) and other instruments to

Method used

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Examples

Experimental program
Comparison scheme
Effect test

no. 1 example

[0010] 1. Operation steps:

[0011] 1.1. Take 19.45mL of 0.2mol / L disodium hydrogen phosphate solution, 0.55mL of 0.1mol / L citric acid solution and 1.32g of sodium tetraphenylborate, mix them with distilled water and dilute to 100mL, and filter with medium-speed filter paper Store it in a brown reagent bottle to obtain sodium tetraphenylborate buffer solution;

[0012] 1.2. Take 5mL of milk to be tested, add dropwise 0.25mL of glacial acetic acid aqueous solution with a volume fraction of 36%, and mix well (the pH value after mixing is about 4.4-4.6). The rotation speed is 4000 rpm, and the duration is 2 minutes to obtain supernatant A;

[0013] 1.3. Take 0.5mL supernatant A, add 0.7mL sodium tetraphenylborate buffer, mix well and centrifuge to get the supernatant (the pH value after mixing is about 4.4-4.6), and the speed of centrifugation is 4000 rpm. The duration is 2 minutes, and the supernatant B is obtained;

[0014] 1.4. Take 1mL supernatant B, add 0.3mL sodium tetra...

no. 2 example

[0024] 1. Operation steps:

[0025] 1.1. Take 19.45mL of 0.2mol / L disodium hydrogen phosphate solution, 0.55mL of 0.1mol / L citric acid solution and 2.0g of sodium tetraphenylborate, mix them with distilled water and dilute to 100mL, filter with medium-speed filter paper and store In the brown reagent bottle, get sodium tetraphenylborate buffer solution;

[0026] 1.2. Take 5mL of milk to be tested, add dropwise 0.25mL of glacial acetic acid aqueous solution with a volume fraction of 36%, mix well (the pH value after mixing is about 4.4-4.6), centrifuge to get the supernatant after coagulation occurs, and centrifuge The rotation speed is 4000 rpm, and the duration is 2 minutes to obtain supernatant A;

[0027] 1.3. Take 0.5mL supernatant A, add 0.7mL sodium tetraphenylborate buffer, mix well and centrifuge to get the supernatant (the pH value after mixing is about 4.4-4.6), and the speed of centrifugation is 4000 rpm. The duration is 2 minutes, and the supernatant B is obtaine...

no. 3 example

[0038] 1. Operation steps:

[0039] 1.1. Take 19.45mL of 0.2mol / L disodium hydrogen phosphate solution, 0.55mL of 0.1mol / L citric acid solution and 1.32g of sodium tetraphenylborate, mix them with distilled water and dilute to 100mL, and filter with medium-speed filter paper Store it in a brown reagent bottle to obtain sodium tetraphenylborate buffer solution;

[0040] 1.2. Take 5mL of the milk to be tested, adjust the pH value to 4.5 with a hydrochloric acid solution with a volume fraction of 37%, and then filter with a medium-speed filter paper to obtain the filtrate;

[0041] 1.3. Take 0.5mL filtrate, add 0.7mL sodium tetraphenylborate buffer, mix well and centrifuge to get the supernatant (the pH value after mixing is about 4.4-4.6), centrifuge at 4000 rpm for 2 minutes to obtain the supernatant;

[0042] 1.4. Take 1mL supernatant, add 0.3mL sodium tetraphenylborate buffer solution, mix well and observe (the pH value after mixing is about 4.4-4.6); if the mixed solution ...

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PUM

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Abstract

The invention provides a method for detecting a potassium salt in cow milk. The method comprises the following steps of mixing 19.45mL of a disodium hydrogen phosphate solution with a concentration of 0.2mol/L, 0.55mL of a citric acid solution with a concentration of 0.1mol/L and 1.32g of sodium tetraphenylborate, dissolving the mixture by distilled water to obtain 100mL of a sodium tetraphenylborate buffer, taking 5mL of cow milk to be detected, dropwisely adding an acid solution into the cow milk, carrying out mixing to obtain a uniform solution, carrying out centrifugation after condensation, collecting the supernatant A, taking 0.5mL of the supernatant A, adding 0.7mL of the sodium tetraphenylborate buffer into the supernatant A, carrying out mixing to obtain a uniform mixture, carrying out centrifugation, collecting the supernatant B, taking 1mL of the supernatant B, adding 0.3mL of the sodium tetraphenylborate buffer into the supernatant B, carrying out mixing to obtain a uniform mixed solution, carrying out observation, wherein if the mixed solution is clear and transparent, the tested cow milk does not contain the potassium salt, and if the mixed solution produces a lot of white precipitates, the tested cow milk contains the potassium salt. The method can be operated simply and has a low detection cost.

Description

technical field [0001] The invention relates to a method for detecting potassium salt in dairy products, and is especially suitable for detecting potassium salt in milk. Background technique [0002] When buying milk, the dry matter content is an important indicator for identifying the quality of milk, so some people will increase the dry matter content of milk by adding potassium salts to the milk, and then it is possible to add water or other substances to the milk for profit. In order to protect the economic interests of enterprises and the food safety of the people, it is of great economic and social value to measure the content of potassium salt in the process of milk purchase. The existing detection methods for potassium salts in dairy products first need to digest the samples, such as wet digestion, microwave digestion and dry ashing, and then use inductively coupled plasma-atomic emission spectrometry (ICP-AES) and other instruments to The detection is not only time...

Claims

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Application Information

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IPC IPC(8): G01N21/82
Inventor 胡彩霞王佳刘美霞任丽李红李秋琴
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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