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Microbial additive for preparing silage

A technology for microbial additives and silage, applied in the field of silage, can solve the problems of large difference in effect, high cost, few types and quantities of additives, etc., and achieve the effects of reducing content, strong acid-producing ability, and prolonging shelf life

Active Publication Date: 2015-02-25
QINGDAO VLAND BIOTECH GRP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, scholars at home and abroad have done a lot of research on silage additives, but the types and quantities of additives that can really be used in production are too small, and the effects vary greatly, and the cost is high. Therefore, more and better additives are further screened. Silage additive is a research hotspot in this field

Method used

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  • Microbial additive for preparing silage
  • Microbial additive for preparing silage

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Screening and identification of embodiment 1 Lactobacillus plantarum ZJY-6

[0021] 1. Strain screening

[0022] 1) Weigh 50 grams of corn stalks that have just been taken out from the silo, and soak them in 100 ml of sterile water for 30 minutes;

[0023] 2) Take the supernatant and dilute it 1000 times with sterile water;

[0024] 3) Primary screening: Spread the diluted supernatant evenly on the MRS plate with 0.5% calcium carbonate, culture at 37°C for 48 hours, separate and cultivate the strains with transparent circles, and obtain 12 strains of lactic acid bacteria, named ZJY-1 respectively , ZJY-2, ..., ZJY-12;

[0025] 4) Re-screening: Inoculate the above 12 strains of lactic acid bacteria into 50mL MRS broth medium respectively, culture at 37°C for 48h, measure the contents of lactic acid, acetic acid and propionic acid in the medium by HPLC, and measure the content of the medium with a pH meter. pH, the specific results are shown in Table 1.

[0026] Table...

Embodiment 3

[0032] Application of embodiment 3 Lactobacillus plantarum ZJY-6 in silage preparation

[0033] 1. Prepare microbial additive samples

[0034] 1) Sample 1: Lactobacillus plantarum ZJY-6, the concentration of live bacteria is 10 8 a / ml;

[0035] 2) Sample 2: Lactobacillus plantarum ZJY-6 and Lactobacillus Brucella were mixed at a ratio of 1:1, and the concentration of the mixed bacteria liquid was 10 8 / ml.

[0036] 3) Sample 3: Lactobacillus plantarum ZJY-6 and Lactobacillus Brucella were mixed according to the live bacteria amount of 1:1, and the concentration of the mixed bacteria solution was 10 8 / ml; cellulase 10000IU / mL, xylanase 10000IU / mL.

[0037] 2. How to use

[0038] 1) Three experimental groups: according to the addition ratio of 250-500mL / ton, spray the same amount of sample 1 (experimental group 1), sample 2 (experimental group 2) and sample 3 (experimental group 3) to 500 kg respectively On the silage raw material (what used in this embodiment is the corn...

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Abstract

The invention relates to the field of silage, and particularly provides a novel lactobacillus plantarum ZJY-6 and a microbial additive containing the lactobacillus plantarum ZJY-6. The lactobacillus plantarum ZJY-6 is preserved in the China Center for Type Culture Collection (CCTCC) of Wuhan University in China On November 19, 2014 and has the preservation number of CCTCC No. M2014579; the strain is high in comprehensive acid production capacity, can be widely used for preparing the silage. After the microbial additive containing the lactobacillus plantarum ZJY-6 is added into the silage, the total content of organic acid in the silage can be effectively increased, the content of ammoniacal nitrogen is reduced, the palatability of the silage can be better improved, and the nutritive value is improved; furthermore, with use of the microbial additive, the content of alcohol is remarkably reduced, the growth of funguses in the silage can be inhibited, and the aerobic stability of the silage is improved, so that the storage life of the silage is prolonged, and the unexpected technical effects can be achieved.

Description

technical field [0001] The invention relates to the field of silage, in particular to a microbial additive for preparing silage. Background technique [0002] Silage is a kind of roughage obtained by chopping green feed with a moisture content of 65%-75%, and inhibiting the reproduction of various bacteria through the fermentation of anaerobic lactic acid bacteria under airtight and hypoxic conditions. . Silage has a sour smell, is soft and juicy, has good palatability, is rich in nutrition, and is good for long-term storage. It is an excellent feed source for livestock. [0003] (1) General silage: the raw materials are chopped, compacted and sealed, and the lactic acid bacteria are multiplied in an anaerobic environment, thereby turning the starch and soluble sugar in the feed into lactic acid. When lactic acid accumulates to a certain concentration, it inhibits the growth of spoilage bacteria and preserves the nutrients in the green feed. [0004] (2) Semi-dry silage (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00
Inventor 张景燕谭乐坤朱彩彩程娇梅唐波刘鲁民
Owner QINGDAO VLAND BIOTECH GRP
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