Secondary proliferation culture method of color osmanthusfragrans test-tube plantlets

Abstract

The present invention relates to a secondary proliferation culture method of color osmanthusfragrans test-tube plantlets. The method comprises: adopting the newly-germinating shoots of the color osmanthusfragrans as the explants, carrying out disinfection sterilization on the explants, cutting into the small segments with the length of 1-1.5 cm under a sterile condition, inoculating into an axillary bud induction culture medium, culturing for 4-5 weeks to produce axillary buds, cutting the axillary buds into the terminal bud or stem segment with the length of 1-1.5 cm, and inoculating into the same culture medium to carry out secondary proliferation culture for 4-5 weeks to obtain the clustered shoots, wherein the culture process is performed under the conditions of the lighting time of 14 h each day, the photosynthetically active photon flux density of 40-50 [mu]mol / m<2>.s and the temperature of 24-26 DEG C, the axillary bud induction culture medium comprises a base culture medium, 0.1-0.5 mg / L of TDZ, 0.1-1.0 mg / L of KT, 5.5 g / L of agar, and 30 g / L of white granulated sugar, and the pH value is adjusted to 5.8. The secondary proliferation culture method of the present invention has characteristics of rapid breeding and no limitation of the season change on the production. In addition, the defects of the cutting seedlings are effectively overcome.

Description

technical field [0001] The invention relates to the technical field of rapid propagation of tissue-cultured test-tube plantlets, in particular to a method for subculture and cultivation of test-tube plantlets of Caigui. Background technique [0002] Caigui is an evergreen small tree or shrub belonging to Oleaceae (Oleaceae). The unique color change of Caigui leaves shows its high ornamental value. As an important garden and economic tree species in my country, it has a broad market prospect. Especially the five-color leaf osmanthus among the colorful osmanthus varieties. [0003] The majority of Osmanthus plants are ornamental flowers and trees and excellent garden tree species in China. Among them, osmanthus is one of the top ten famous flowers in China, with good shape, color, fragrance and rhyme, and has high ornamental value. At present, there are varieties of Caiyegui in China, such as Yuntian Caigui and Yinbian Caigui. The leaves are finally in the state of mosaic lea...

AI Technical Summary

Problems solved by technology

[0004] The propagation of five-color osmanthus generally adopts twig cuttings. The production is limited by seasonal changes and its cutting propagation requires a large number of cuttings. Due to the inconsistency of cutting sources, the growth uniformity of cutting seedlings may be worse than that of tissue culture seedlings, etc.

[0005] Rapid propagation technology has been widely used in the field of agricultural technology, but for each variety of plants, the technical methods vary widely, and there is no report on the rapid propagation technology of five-color leaf osmanthus test tube seedlings using tissue culture technology

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