Serum total bile acid detection reagent with stable and strong anti-interference capability
A technology of total bile acids and detection reagents, which is applied in the direction of material analysis by observing the influence on chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem of unsatisfactory detection effect, reagent stability and anti-interference ability. Not easy to chelate salt ions, improve stability and anti-interference ability, enhance antistatic and antibacterial properties
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Embodiment 1
[0058] A stable and strong anti-interference detection reagent for total bile acid, comprising reagent R1 and reagent R2.
[0059] Wherein, the composition of reagent R1 is :
[0060] MES (2-(N-morpholino)ethanesulfonic acid) buffer solution (pH4.5) 80mmol / L
[0061] thioNAD + 0.6g / L
[0062] BSA 0.8g / L
[0063] Sucrose 8g / L
[0064] Mannitol 8g / L
[0065] Alkyl glucoside (APG) 0.6g / L
[0066] Cocamidopropyl Betaine 0.6g / L
[0067] Sodium azide (NaN 3 ) 0.8g / L;
[0068] Reagent R2 consists of:
[0069] HEPES (4-hydroxyethylpiperazineethanesulfonic acid) buffer solution (pH9.2) 150mmol / L
[0070] 3α-Hydroxysteroid dehydrogenase (3α-HSD) 10KU / L
[0071] NADH 2g / L
[0072] BSA 0.6g / L
[0073] Nanoparticles 10mmol / L
[0074] Sucrose 8g / L
[0075] Mannitol 8g / L
[0076] Alkyl glucoside (APG) 0.6g / L
[0077] Cocamidopropyl Betaine 0.6g / L
[0078] Sodium azide (NaN 3 ) 0.8g / L.
[0079] The above-mentioned nanoparticles are ...
Embodiment 2
[0081] A stable and strong anti-interference detection reagent for total bile acid, comprising reagent R1 and reagent R2.
[0082] Wherein, the composition of reagent R1 is :
[0083] MES (2-(N-morpholino)ethanesulfonic acid) buffer solution (pH4.5) 150mmol / L
[0084] thioNAD + 1.2g / L
[0085] BSA 1.2g / L
[0086] Sucrose 15g / L
[0087] Mannitol 15g / L
[0088] Alkyl glucoside (APG) 2g / L
[0089] Cocamidopropyl Betaine 2g / L
[0090] Sodium azide (NaN 3 ) 1.5g / L;
[0091] Reagent R2 consists of:
[0092] HEPES (4-hydroxyethylpiperazineethanesulfonic acid) buffer solution (pH9.2) 300mmol / L
[0093] 3α-hydroxysteroid dehydrogenase (3α-HSD) 20KU / L
[0094] NADH 5g / L
[0095] BSA 1.2g / L
[0096] Nanoparticles 30mmol / L
[0097] Sucrose 15g / L
[0098] Mannitol 15g / L
[0099] Alkyl glucoside (APG) 2g / L
[0100] Cocamidopropyl Betaine 2g / L
[0101] Sodium azide (NaN 3 ) 1.5g / L.
[0102] The above-mentioned nanoparticles are gam...
Embodiment 3
[0104] A stable and strong anti-interference detection reagent for total bile acid, comprising reagent R1 and reagent R2.
[0105] Wherein, the composition of reagent R1 is :
[0106] MES (2-(N-morpholino)ethanesulfonic acid) buffer solution (pH4.5) 100mmol / L
[0107] thioNAD + 0.8g / L
[0108] BSA 1.0g / L
[0109] Sucrose 10g / L
[0110] Mannitol 10g / L
[0111] Alkyl glucoside (APG) 1g / L
[0112] Cocamidopropyl Betaine 1g / L
[0113] Sodium azide (NaN 3 ) 1g / L.
[0114] Each component in the reagent R2 is:
[0115] HEPES (4-hydroxyethylpiperazineethanesulfonic acid) buffer solution (pH9.2) 200mmol / L
[0116] 3α-Hydroxysteroid dehydrogenase (3α-HSD) 15KU / L
[0117]NADH 3g / L
[0118] BSA 1g / L
[0119] Nanoparticles 20mmol / L
[0120] Sucrose 10g / L
[0121] Mannitol 10g / L
[0122] Alkyl glucoside (APG) 1g / L
[0123] Cocamidopropyl Betaine 1g / L
[0124] Sodium azide (NaN 3 ) 1g / L.
[0125] The above-mentioned nanoparticles a...
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