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Serum total bile acid detection reagent with stable and strong anti-interference capability

A technology of total bile acids and detection reagents, which is applied in the direction of material analysis by observing the influence on chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem of unsatisfactory detection effect, reagent stability and anti-interference ability. Not easy to chelate salt ions, improve stability and anti-interference ability, enhance antistatic and antibacterial properties

Active Publication Date: 2015-02-18
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many types of reagents for the detection of total bile acids by circulation method in the market, but the stability and anti-interference ability of the reagents are generally not good, so the detection effect is not ideal

Method used

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  • Serum total bile acid detection reagent with stable and strong anti-interference capability
  • Serum total bile acid detection reagent with stable and strong anti-interference capability
  • Serum total bile acid detection reagent with stable and strong anti-interference capability

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] A stable and strong anti-interference detection reagent for total bile acid, comprising reagent R1 and reagent R2.

[0059] Wherein, the composition of reagent R1 is :

[0060] MES (2-(N-morpholino)ethanesulfonic acid) buffer solution (pH4.5) 80mmol / L

[0061] thioNAD + 0.6g / L

[0062] BSA 0.8g / L

[0063] Sucrose 8g / L

[0064] Mannitol 8g / L

[0065] Alkyl glucoside (APG) 0.6g / L

[0066] Cocamidopropyl Betaine 0.6g / L

[0067] Sodium azide (NaN 3 ) 0.8g / L;

[0068] Reagent R2 consists of:

[0069] HEPES (4-hydroxyethylpiperazineethanesulfonic acid) buffer solution (pH9.2) 150mmol / L

[0070] 3α-Hydroxysteroid dehydrogenase (3α-HSD) 10KU / L

[0071] NADH 2g / L

[0072] BSA 0.6g / L

[0073] Nanoparticles 10mmol / L

[0074] Sucrose 8g / L

[0075] Mannitol 8g / L

[0076] Alkyl glucoside (APG) 0.6g / L

[0077] Cocamidopropyl Betaine 0.6g / L

[0078] Sodium azide (NaN 3 ) 0.8g / L.

[0079] The above-mentioned nanoparticles are ...

Embodiment 2

[0081] A stable and strong anti-interference detection reagent for total bile acid, comprising reagent R1 and reagent R2.

[0082] Wherein, the composition of reagent R1 is :

[0083] MES (2-(N-morpholino)ethanesulfonic acid) buffer solution (pH4.5) 150mmol / L

[0084] thioNAD + 1.2g / L

[0085] BSA 1.2g / L

[0086] Sucrose 15g / L

[0087] Mannitol 15g / L

[0088] Alkyl glucoside (APG) 2g / L

[0089] Cocamidopropyl Betaine 2g / L

[0090] Sodium azide (NaN 3 ) 1.5g / L;

[0091] Reagent R2 consists of:

[0092] HEPES (4-hydroxyethylpiperazineethanesulfonic acid) buffer solution (pH9.2) 300mmol / L

[0093] 3α-hydroxysteroid dehydrogenase (3α-HSD) 20KU / L

[0094] NADH 5g / L

[0095] BSA 1.2g / L

[0096] Nanoparticles 30mmol / L

[0097] Sucrose 15g / L

[0098] Mannitol 15g / L

[0099] Alkyl glucoside (APG) 2g / L

[0100] Cocamidopropyl Betaine 2g / L

[0101] Sodium azide (NaN 3 ) 1.5g / L.

[0102] The above-mentioned nanoparticles are gam...

Embodiment 3

[0104] A stable and strong anti-interference detection reagent for total bile acid, comprising reagent R1 and reagent R2.

[0105] Wherein, the composition of reagent R1 is :

[0106] MES (2-(N-morpholino)ethanesulfonic acid) buffer solution (pH4.5) 100mmol / L

[0107] thioNAD + 0.8g / L

[0108] BSA 1.0g / L

[0109] Sucrose 10g / L

[0110] Mannitol 10g / L

[0111] Alkyl glucoside (APG) 1g / L

[0112] Cocamidopropyl Betaine 1g / L

[0113] Sodium azide (NaN 3 ) 1g / L.

[0114] Each component in the reagent R2 is:

[0115] HEPES (4-hydroxyethylpiperazineethanesulfonic acid) buffer solution (pH9.2) 200mmol / L

[0116] 3α-Hydroxysteroid dehydrogenase (3α-HSD) 15KU / L

[0117]NADH 3g / L

[0118] BSA 1g / L

[0119] Nanoparticles 20mmol / L

[0120] Sucrose 10g / L

[0121] Mannitol 10g / L

[0122] Alkyl glucoside (APG) 1g / L

[0123] Cocamidopropyl Betaine 1g / L

[0124] Sodium azide (NaN 3 ) 1g / L.

[0125] The above-mentioned nanoparticles a...

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Abstract

The invention discloses a serum total bile acid detection reagent with stable and strong anti-interference capability and belongs to the technical field of clinical in-vitro detection. The serum total bile acid detection reagent adopts a novel MES and HEPES biological buffer system, and a novel ampholytic surfactant cocamidopropyl betaine and a non-ionic surface active agent alkyl glycoside are added, so that the anti-interference capability and stability of the serum total bile acid detection reagent are greatly improved; besides, gamma-Fe2O3 nano particles are also added in a component R2 of the serum total bile acid detection reagent, and the gamma-Fe2O3 nano particles have a special nanometer effect and biological enzyme activity characteristics, so that activities of NADH and 3alpha-HSD enzyme in the serum total bile acid detection reagent can be effectively improved, reaction is catalyzed, and the accuracy degree of analysis and detection is improved. Therefore the serum total bile acid detection reagent can be used for detecting the content of serum total bile acid and has strong anti-interference capability and good stability.

Description

technical field [0001] The invention relates to a reagent for clinical determination of total bile acid content in serum, belonging to the technical field of clinical in vitro detection. Background technique [0002] Total bile acids (TBA) are synthesized in the liver and combined with glycine or taurine to form conjugated bile acids, which are then secreted into bile by liver cells. After the bile reaches the intestinal tract, they are hydrolyzed into free forms by bacteria in the intestinal tract 97% of bile acids are reabsorbed by the intestinal tract and then returned to the liver, so that the cycle continues, so that the total bile acids can exert the maximum physiological effect, and it can also prevent the total bile acids from entering the circulation in large quantities and causing damage to other tissue cells (total The pH of bile acids is very low). The concentration of bile acid in normal human blood is very low and relatively stable. Hepatocyte Lesions occur, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 杨君莉李志明甘宜梧谭柏清谢清华包兴艳赵新
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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