Method for measuring response function of time resolution fluorescence instrument in yellow-green optical band
A technology of instrument response function and measurement time, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve problems such as instrument response function system error, and achieve the effects of improving accuracy, large quantum yield, and easy preparation
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[0029] The specific implementation manner of the present invention will be further described in detail below in conjunction with the accompanying drawings and examples, but the protection scope of the present invention should not be limited thereby.
[0030] Preparation of reference dyes
[0031] First prepare the disodium hydrogen phosphate-sodium hydroxide buffer solution with pH 10, weigh 18.3g of sodium iodide (NaI), dilute it to 10mL with the prepared pH 10 buffer solution, and prepare 12.2M (nearly saturated) NaI Solution; weigh 1.91g of sodium fluorescein, and dilute to 100mL with the prepared pH10 buffer solution to prepare a 51.4mM sodium fluorescein alkaline solution; measure the prepared 12.2M NaI (sodium iodide) solution 1000 μL, 10 μL of 51.44 mM sodium fluorescein solution and mix well, the obtained mixed solution is the reference dye for measuring the instrument response function of time-resolved fluorescence, wherein the final concentration of iodide ion as a...
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