A kind of preparation method of one-component tmb chromogenic solution
A color developing solution, one-component technology, applied in material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problems of troublesome operation, high color background, poor stability, etc. problems, to achieve the effect of improving efficiency, simplifying the operation process, and improving stability
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Embodiment 1
[0023] A kind of preparation method of one-component TMB chromogenic solution, comprises the following steps:
[0024] First, weigh 7g of citric acid, 16g of disodium hydrogen phosphate, and 0.1g of sodium perborate into a 1000mL beaker A, and add 800mL of purified water to dissolve;
[0025] Next, adjust the pH of the solution in beaker A to 4.5, add 20 mL of glycerin, and then add 1 EDTA;
[0026] Then, weigh 5mg of TMB and place it in a 100mL beaker B, add 10ml of ethanol to dissolve it;
[0027] Finally, transfer the solutions in beaker A and beaker B to a 1000mL volumetric flask, add purified water to make up to 1000mL, and shake well.
Embodiment 2
[0029] A kind of preparation method of one-component TMB chromogenic solution, comprises the following steps:
[0030] First, weigh 10g of citric acid, 20g of disodium hydrogen phosphate, and 0.5g of sodium perborate into a 1000mL beaker A, and add 800mL of purified water to dissolve;
[0031] Next, adjust the pH of the solution in beaker A to 6.0, add 30 mL of glycerin, and then add 3 g of EDTA;
[0032] Then, weigh 40 mg of TMB and place it in a 100 mL beaker B, add 30 ml of ethanol to dissolve it;
[0033] Finally, transfer the solutions in beaker A and beaker B to a 1000mL volumetric flask, add purified water to make up to 1000mL, and shake well.
Embodiment 3
[0035] A kind of preparation method of one-component TMB chromogenic solution, comprises the following steps:
[0036] First, weigh 9g of citric acid, 18g of disodium hydrogen phosphate, and 0.2g of sodium perborate into a 1000mL beaker A, and add 800mL of purified water to dissolve;
[0037] Next, adjust the pH of the solution in beaker A to 5.0, add 25 mL of glycerin, and then add 2 g of EDTA;
[0038] Then, weigh 30 mg of TMB and place it in a 100 mL beaker B, add 20 ml of ethanol to dissolve it;
[0039] Finally, transfer the solutions in beaker A and beaker B to a 1000mL volumetric flask, add purified water to make up to 1000mL, and shake well.
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