Segmenting method of gecko brain atlas processed through hematoxylin-eosin (HE) staining

Abstract

The invention relates to a segmenting method of a gecko brain atlas processed through hematoxylin-eosin (HE) staining. Aimed at color features of tissues on the brain atlas shot by a Microscope or a digital camera, the segmenting method based on an YCbCr space is provided. According to the segmenting method of the gecko brain atlas processed through the HE staining, firstly, the color of the atlas is converted from an RGB space into the YCbCr space, then the brain atlas is segmented according to distribution differences of components Cb and components Cr in different regions of the atlas, and finally the different tissues in the atlas are completely segmented. The segmenting method provides a more convenient condition for researches on the brain structures of geckos and corresponding biobots.

Description

technical field [0001] The invention relates to a segmentation method of an HE-stained gecko brain atlas, which belongs to the field of image processing. Background technique [0002] Since the 9 / 11 incident, all parts of the world have increased investment in anti-terrorism, especially the research on anti-terrorism robots. The progress in this area will further promote the progress of anti-terrorism technology. At present, the widely used mechanical robot has developed to a certain position, but its shortcomings are also obvious, that is, the energy it can carry is very limited, and it is easy to be found. In view of such drawbacks, another robot is currently being developed Quietly rising, that is, bio-robots. By inserting electrodes into a specific area of ​​the brain of the creature and discharging it, the creature can be controlled to move according to the specified command. It can supplement its own energy by looking for external food, and it is difficult to find. B...

AI Technical Summary

Problems solved by technology

At present, the usual research method is nuclear magnetic resonance, but its shortcomings are obvious. The amount of information provided by its images is not very rich, mainly due to the monotony of its color, especially for small tissues such as the gecko brain. The amount of information that can be provided is even more limited. In order to break through the limitations of the MRI method, the brain tissue is made into paraffin sections, and different tissues can be displayed in different colors through HE staining.

It is photographed by a microscope digital camera, and various tissues are mixed together in the obtained map. In order to study different tissues, different tissues must be distinguished. Since the light cannot be kept constant during the shooting process at different times, the light The change of the color will lead to a certain change in the color components of the captured atlas, which often brings difficulties to the segmentation of different regions of the atlas. The traditional segmentation method based on RGB space will appear to be incomplete or over-segmented here. , so there is an urgent need for a new segmentation method that can thoroughly and effectively segment the brain map of the gecko

Images