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Multifunctional photoelectrochemical sensor based on G-tetrahedron/hemin

A photoelectrochemical, tetrahedral technology, applied in the direction of material electrochemical variables, etc.

Active Publication Date: 2015-01-21
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To the best of our knowledge, so far, there have been no reports of photoelectrochemical measurements based on PbX (X stands for sulfur, selenium, tellurium) quantum dots

Method used

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  • Multifunctional photoelectrochemical sensor based on G-tetrahedron/hemin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] a, in containing 0.045mmol cysteine, 0.015mmol mercaptohexanol solution and 0.015mmol Pb(CH 3 COO) 2 In the solution, add 1mol / L NaOH solution to adjust the pH of the solution to 9. After passing through high-purity nitrogen for 30 minutes, add 15mL of 0.01mol / L NaHSe aqueous solution, continue to pass through nitrogen and stir at 60 degrees for 2 hours to obtain water-soluble PbSe quantum dots;

[0022] b. Immerse the pretreated ITO glass sheet in a solution of 2% PDDA polymer containing 0.1mmol / L NaCl, rinse the electrode surface with deionized water after 10 minutes; then immerse it in the water-soluble PbSe quantum dot solution After 10 minutes, rinse the electrode surface with deionized water. The above steps can modify the PbSe quantum dots on the ITO electrode.

[0023] c. Immerse the PbSe quantum dot modified electrode in 1mL aqueous solution containing 20mg EDS and 10mg NHS to react for 1 hour, and then use the cleaning solution (0.01mol / L Tris-HCl buffer so...

Embodiment 2

[0026] a, 17μL thioglycolic acid, 0.01mmol mercaptohexanol solution and 0.015mmol Pb(CH 3 COO) 2 After the solution is mixed, add 1mol / L NaOH solution to adjust the pH of the solution to 11, and after feeding high-purity nitrogen for 30min, add 20mL 0.01mol / L NaOH solution 2 S aqueous solution, continue stirring and reacting at 90 degrees under nitrogen gas for 4 hours, to obtain water-soluble PbS quantum dots;

[0027] b. Immerse the pretreated ITO glass sheet in a solution of 2% PDDA polymer containing 0.1mmol / L NaCl, rinse the electrode surface with deionized water after 10 minutes; then immerse it in the water-soluble PbS quantum dot solution After 10 minutes, rinse the electrode surface with deionized water. The above steps can modify the PbS quantum dots on the ITO electrode.

[0028] c. Immerse the PbS quantum dot modified electrode in 1mL aqueous solution containing 20mg EDS and 10mg NHS to react for 1 hour, and then use the cleaning solution (0.01mol / L Tris-HCl buf...

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Abstract

The invention relates to a novel and multifunctional photoelectrochemical sensor which is capable of triggering the light current of a water-soluble PbX (X represents sulfur or selenium) quantum dot to be increased based on G-tetrahedron / hemin. The G-tetrahedron / hemin is formed on the surface of the PbX (X represents sulfur or selenium) quantum dot by the specific identification reaction between a target object to be measured and nucleic acid recognition molecules fixed on the surface of an electrode. The G-tetrahedron / hemin compound is taken as an electron acceptor of the quantum dot and is used for carrying out catalytic reduction on dissolved oxygen, so that the separation efficiency of the photo-induced charges of the quantum dot can be improved, and the light current of the PbX (X represents sulfur or selenium) quantum dot can be increased. According to the multifunctional photoelectrochemical sensor, multiple target objects (such as DNA, thrombin, triphosadenine, adenosine monophosphate and lead (II) ions) are unmarked by selecting nucleic acid probes with different sequences; the multifunctional photoelectrochemical sensor is simple, convenient and high in sensitivity and selectivity when being used for measurement.

Description

Technical field: [0001] The invention relates to the field of nanometer analysis and detection, in particular to the application of water-soluble PbX (X represents sulfur and selenium) quantum dots as modified electrodes in photoelectrochemical analysis. Background technique: [0002] It is an urgent task for analytical chemistry to establish a rapid detection method for target molecules with high sensitivity, high throughput and strong specificity. Deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) not only store and transmit genetic information, but also interact with other types of molecules through their own spatial structure. Nucleic acid aptamers (aptamers) are screened out by Systematic Evolution of Ligands by Exponential enrichment (SELEX). Nucleic acid fragments (single-stranded DNA or RNA), which have high affinity and specific recognition ability for target molecules [Goulko A A, Li F, Le X C. Trends Anal. Chem., 2009, 28: 878-892.] . Aptamers do not depend...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30
Inventor 王光丽束军仙董玉明杨亚鑫张颖刘志伟
Owner JIANGNAN UNIV
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