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Centella extract as well as preparation and application thereof

A centella asiatica extract and the technology of centella asiatica, applied in the field of centella asiatica extract and its preparation and application, can solve the problems of high price and high cost, and achieve the effects of low cost, promoting cell proliferation and high efficiency

Inactive Publication Date: 2015-01-14
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It has been documented in the literature that Centella asiatica extract promotes the differentiation of neuron model SH-SY5Y cells in the presence of nerve growth factor Nerve Growth Factor (NGF), but there are also some defects. Presupposes expensive nerve growth factor, so the cost is high

Method used

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  • Centella extract as well as preparation and application thereof
  • Centella extract as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Immerse Centella asiatica in 75% ethanol solution, add 10mL ethanol solution per 1 gram of Centella asiatica, extract twice with ultrasound, each time for 1 hour, combine the extracts, concentrate under reduced pressure until there is no alcohol smell, centrifuge (8000 rpm minutes, 15 minutes), discard the precipitate, and prepare for loading.

[0032] Weigh the AB-8 resin with the same weight as the raw material of Centella asiatica, wash with alcohol and wash with water, then pack into the column, add water to wash the column until the column no longer contains air bubbles.

[0033] Load the sample, let it stand for 1 hour for adsorption, and then elute in the order of water, 20% ethanol, 50% ethanol, 75% ethanol, and 95% ethanol, and receive the extract at a flow rate of 100ml for 5 minutes. Use 3-column, 5-column, 3-column, 3-column, and 2-column volume solutions for elution respectively (Note: The volume of the resin is one column). The resulting eluate was concen...

Embodiment 2

[0034] Embodiment 2 drug effect comparative experiment

[0035] The PC12 cells were seeded in a 96-well plate, and the cck-8 toxicity test was performed to obtain the dosage of the drug. Afterwards, PC12 cells were inoculated on a 12-well plate, and after drug incubation for 72 hours, the synapse length of PC12 cells was observed, photographed under a microscope, and data statistical analysis was performed with software, compared with the normal group, the best drug effect was obtained Column 2 with 75% ethanol and next best column 3 with 50% ethanol. The results of comparing the second column eluted with 75% ethanol with the normal PC12 without Centella asiatica extract are as follows: figure 1 as shown, figure 1 Among them, 0 represents the normal group without Centella asiatica extract, 1:100 represents the treatment group in which the second column Centella Asiatica extract eluted with 75% ethanol was diluted 100 times, and 1:50 represents the treatment group that was wa...

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Abstract

The invention discloses a centella extract. The preparation method comprises the following steps: with an ethanol solution as a solvent, carrying out crude extraction on centella by using an ultrasonic extraction method; and with an AB-8 resin separation column and the ethanol solution as eluents, separating out effective components, wherein the target components eluted by 30%-50% of ethanol and 51%-75% of ethanol solution are the centella extracts. The centella extract disclosed by the invention is capable of promoting cell proliferation and differentiation of a neuron model PC12 without NFG induction, and can be applied to treatment of depressive disorder, and related diseases of various nerve injuries and neuronal apoptosis. The extraction method provided by the invention is simple in process, low in cost and high in efficiency.

Description

technical field [0001] The invention relates to the preparation and application of a centella asiatica extract. Background technique [0002] According to World Health Organization estimates, by 2030, depression will be the second most common cause of disability and death after heart disease. A 2008 report by the Organization for Economic Co-operation and Development noted that depression is one of the costliest mental illnesses and that it affects the workforce in high numbers. In the European Union, the costs associated with depression exceed 118 billion euros (958 billion yuan). This is equivalent to 1% of the gross regional product of the entire EU. Therefore, it is of great social and economic significance to strengthen the prevention and treatment of depression and find active and effective treatment methods. [0003] The most widely clinically used antidepressants, such as: selective 5-HT reuptake inhibitors (SSRI), 5-HT / norepinephrine (NE) reuptake inhibitors (SNR...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/23A61P25/24A61P25/00
CPCA61K36/23A61K2236/333A61K2236/51A61K2236/53
Inventor 姜慧吕君蔚丁显廷林津津
Owner SHANGHAI JIAO TONG UNIV
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