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Rapid propagation method of lindera communis

A fragrant and fast technology, applied in the field of plants, can solve the problems of unmanned research and achieve the effects of high survival rate, low energy consumption and simple operation

Inactive Publication Date: 2015-01-07
NANJING TONGZE AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tissue culture method can overcome the limitation of time and has a high reproduction rate, but no one has studied it so far

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Take the plump seeds of the geranium, soak them in bleaching powder for 3 minutes, wash them with running water for 1 hour, treat them with 70% alcohol on a clean bench for 30 seconds, treat them with mercuric chloride for 15 minutes, rinse them with sterile water 5-7 times, insert the sterilized seeds Cultivate in MS medium, add sucrose 30g / L, agar 7.5g / L, pH5, light 2000lx, temperature 25°C, take the grown aseptic seedlings of Cedarwood, cut off the root, and put it into the medium to improve DKW+6 -BA2.0mg / L+IBA0.1mg / L+PVP3g / L add sucrose 30g / L, agar 7.5g / L, PH5, light 2000lx, put the clustered buds cultured by proliferation into rooting medium 1 / 2DKW+IBA0. 1mg / L+ diconazole 0.3mg / L+AgNO 3 4mg / L, add sucrose 20g / L, agar 7.5g / L, PH5, light 3000lx, when the test-tube plantlet is about 5cm long, take the test-tube plantlet out of the culture bottle, wash off the root medium, and plant it in perlite: Sandy loam soil = 1:2 is placed in a greenhouse for cultivation. After...

Embodiment 2

[0011] Take the plump seeds of the geranium, soak them in bleaching powder for 3 minutes, wash them with running water for 1 hour, treat them with 70% alcohol on a clean bench for 30 seconds, treat them with mercuric chloride for 15 minutes, rinse them with sterile water 5-7 times, insert the sterilized seeds Cultivate in WPM medium, add 30g / L sucrose, 7.5g / L agar, PH5, light 2000lx, temperature 25°C, take the grown aseptic seedlings of Cedarwood, cut off the root, and put it into the medium to improve DKW+6 -BA2.0mg / L+IBA0.1mg / L+PVP3g / L add sucrose 30g / L, agar 7.5g / L, PH5, light 2000lx, put the clustered buds cultured by proliferation into rooting medium 1 / 2DKW+IBA0. 3mg / L+Lenconazole 0.5mg / L+AgNO 3 4mg / L, add sucrose 20g / L, agar 7.5g / L, PH5, light 3000lx, when the test-tube plantlet is about 5cm long, take the test-tube plantlet out of the culture bottle, wash off the root medium, and plant it in perlite: Sandy loam soil = 1:2 is placed in a greenhouse for cultivation. Afte...

Embodiment 3

[0013] Take the plump seeds of the geranium, soak them in bleaching powder for 3 minutes, wash them with running water for 1 hour, treat them with 70% alcohol on a clean bench for 30 seconds, treat them with mercuric chloride for 15 minutes, rinse them with sterile water 5-7 times, insert the sterilized seeds Cultivate in MS medium, add sucrose 30g / L, agar 7.5g / L, pH5, light 2000lx, temperature 25°C, take the grown aseptic seedlings of Cedarwood, cut off the root, and put it into the medium to improve DKW+6 -BA2.0mg / L+IBA0.1mg / L+PVP3g / L add sucrose 30g / L, agar 7.5g / L, PH5, light 2000lx, put the clustered buds cultured by proliferation into rooting medium 1 / 2DKW+IBA0. 2mg / L+ diconazole 0.3mg / L+AgNO 3 4mg / L, add sucrose 20g / L, agar 7.5g / L, PH5, light 3000lx, when the test-tube plantlet is about 5cm long, take the test-tube plantlet out of the culture bottle, wash off the root medium, and plant it in perlite: Sandy loam soil = 1:2 is placed in a greenhouse for cultivation. After...

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PUM

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Abstract

The invention relates to a rapid propagation method of lindera communis. The rapid propagation method comprises the steps of obtaining of aseptic seedlings, induction of cluster buds, multiplication of cluster buds, rooting induction, and acclimatization and transplanting. The rapid propagation method of lindera communis is simple and convenient to operate, and the lindera communis is high in growth coefficient, high in survival rate and stable in inheritable characters; as a result, the rapid propagation method is capable of obtaining a large quantity of lindera communis seedlings in short time and lays a foundation for large-scale development and effective utilization of lindera communis.

Description

technical field [0001] The present invention relates to the cultivation of chrysanthemum under the condition of tissue culture, and belongs to the technical field of plants. Background technique [0002] Sweet leaf tree, evergreen shrub or small tree plant, Lauraceae, Lindera communis Hemsl. , Alias: Fragrant fruit tree, fine-leaf false camphor, Qianjin incense, Qianjin tree, wild ginger, fragrant leaves, big fragrant leaves. It grows in sparse forests at the bottom of hills and mountains. Like acidic soil, resistant to pruning. Distributed in Shaanxi, Gansu, Hunan, Hubei, Jiangxi, Zhejiang, Fujian, Taiwan, Guangdong, Guangxi, Yunnan, Guizhou, Sichuan and other provinces. It is also distributed in Indochina Peninsula. The branches, leaves or stem bark of the plant can be used for medicinal purposes. It comes from the "Guangxi Medicinal Plant List". It can be harvested throughout the year. The bark should be scraped off and dried in the sun. It has the effects of detoxif...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G1/00
Inventor 杨存
Owner NANJING TONGZE AGRI SCI & TECH
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