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New fermentation technology of sodium glutamate

A technology of sodium glutamate and fermentation process, which is applied in biochemical equipment and methods, water/sludge/sewage treatment, microorganisms, etc. , the effect of large biomass, reducing operating costs

Active Publication Date: 2014-12-17
XINJIANG FUFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the traditional process, the present invention provides a method of using sodium hydroxide instead of liquid ammonia to neutralize the fermentation broth and directly produce sodium glutamate, so as to reduce the cost of fermentation raw materials and solve the problem of using a large amount of acid in the separation process of subsequent extraction. to solve the pollution problem in the production process of sodium glutamate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Carry out fermentation with glucose as raw material, wherein the glucose is 1 ton, then add liquid ammonia and sodium hydroxide solution to the fermentation liquid (glutamic acid content is 11.9%), the molar ratio of liquid ammonia and sodium hydroxide is 2:1 , obtain the fermented liquid that contains the crude product of sodium glutamate that pH is 6.9, the two add simultaneously, the pH value of fermented liquid is 6.9, obtain the fermented liquid that contains the crude product of sodium glutamate, then will contain the crude product of sodium glutamate The fermented liquid of the product is filtered through a plate frame or a micro-membrane to remove bacterial protein. The pore size of the micro-membrane is 0.04um, centrifuged at 2000r / min for 3 minutes, and the sterilized liquid is collected. The sterilizing solution is pumped into the decolorizing tank for decolorization treatment, and powdered activated carbon with a mass of 1.5% of the sterilizing solution is ad...

Embodiment 2

[0050] Carry out fermentation with glucose as raw material, wherein the glucose is 1 ton, then add liquid ammonia and sodium hydroxide solution in the fermented liquid, the molar ratio of liquid ammonia and sodium hydroxide is 2: 1, obtain the pH is 7.1 and contain glutamine The fermented liquid of sodium glutamate crude product, both add simultaneously, and the add-on of liquid ammonia and sodium hydroxide solution is adjusted according to the acid production progress of fermented liquid, the pH value of stable fermented liquid is 6.9, obtains the crude product containing sodium glutamate Then filter the fermentation broth containing the crude product of sodium glutamate through a plate frame or micro-membrane to remove bacterial protein, the micro-membrane pore size is 0.06 μm, and centrifuge at 2000r / min for 5 minutes to collect the sterilizing solution. The sterilizing solution is pumped into the decolorizing tank for decolorization treatment, and powdered activated carbon ...

Embodiment 3

[0058] Get Xinjiang Fufeng monosodium glutamate production fermentation workshop waste water, enter the sewage treatment system according to the process still bottom material of embodiment 1, utilize 50L bucket as test equipment and band stirring, get 30L respectively, add in two buckets, adjust pH to be 7.0, water temperature 20 ℃, sampling and measuring COD, ammonia nitrogen, and total nitrogen data; the control group did not add the compound bacterial agent, and the experimental group added the compound bacterial agent in Example 1, and added 30 grams of microbial preparations per cubic meter of wastewater each time, and added once a day After one week of continuous dosing, samples were taken to measure COD, ammonia nitrogen, and total nitrogen data. After treatment, the COD of the experimental group was 21mg / L, and the NH3-N was 3mg / L. The treated wastewater fully meets the discharge standard.

[0059] COD average removal rate Ammonia nitrogen average removal...

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PUM

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Abstract

The invention relates to a new fermentation technology of sodium glutamate. The technology includes following steps: adding liquid ammonia and sodium hydroxide to a fermented solution of sodium glutamate at the same time for carrying out a reaction with a molar ratio of the liquid ammonia to the sodium hydroxide being 2:1 to obtain a fermenting solution which contains a crude product of the sodium glutamate and is 6.9-7.1 in pH value; performing micro-membrane filtration, wherein hole diameters of the micro membrane are 0.04-0.06 [mu]m; performing centrifugation at 2000 r / min for 3-5 min; collecting a microorganism-removed solution and a precipitated bacterial protein; pumping the microorganism-removed solution to a decoloring pot for carring out a decoloration treatment; adding powdery activated carbon accounting for 1.5% of the mass of the microorganism-removed solution to the decoloring pot; controlling the temperature in the decoloring pot within 45-50 DEG C; performing concentration after the decoloration treatment being carried out for 30 min, wherein concentration parameters are described as follows: a temperature is 60-70 DEG C and a vacuum degree is -0.1 kpa; performing one-step crystallization to obtain a crude crystal of sodium glutamate; and performing purification and treating wastewater. The technology is economical and environmental protective and has a wide application prospect.

Description

technical field [0001] The invention relates to the field of extraction technology of sodium glutamate in the biological fermentation industry, and specifically provides a new fermentation technology of sodium glutamate. Background technique [0002] The chemical name of sodium glutamate is monosodium d-aminoglutarate, which is a salt formed by sodium ions and glutamate ions, wherein glutamic acid is an amino acid, and sodium is a metal element. The main ingredient of seasoning MSG commonly used in life is sodium glutamate. Monosodium glutamate is a commonly used flavoring agent in daily life, which can increase the umami taste of food and help improve the digestibility of food by the human body. In addition, sodium glutamate has very important functions and is widely used in food, medicine, industry and agriculture. [0003] The following problems exist in the production process of sodium glutamate at present: [0004] On the one hand: During the fermentation process, as...

Claims

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Application Information

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IPC IPC(8): C07C229/24C12R1/685C12N1/20C12N1/14C12R1/11C12R1/01C07C227/18C12R1/645C02F3/34C07C227/40
Inventor 潘悦洪刘守卫肖勇董爽
Owner XINJIANG FUFENG BIOTECH
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