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Dual-emission ratio-type quantum dot fluorescence probe, preparation method and application thereof

A technology of fluorescent probes and quantum dots, applied in the field of dual-emission ratiometric quantum dot fluorescent probes and its preparation, can solve the problems of cumbersome sample pretreatment, time-consuming, poor selectivity, etc.

Inactive Publication Date: 2014-12-10
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of double-emission ratiometric quantum dot fluorescent probe and its preparation method for visual detection of aspirin, to overcome the large amount of solvent consumption in the detection of aspirin process in the prior art, time-consuming, loaded down with trivial details sample pretreatment and Disadvantages such as poor selectivity

Method used

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  • Dual-emission ratio-type quantum dot fluorescence probe, preparation method and application thereof
  • Dual-emission ratio-type quantum dot fluorescence probe, preparation method and application thereof

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Effect test

Embodiment 1

[0030] (1) 30.3 mg sodium borohydride (NaBH 4 ) and 51.04 mg of tellurium powder were added to the centrifuge tube, and then 3.0 mL of twice-distilled water was added to dissolve the solid completely; the centrifuge tube was placed in an ultrasonic machine for ultrasonic reaction, and the mouth of the tube was kept to vent gas, and the final white liquid was the desired The precursor NaHTe solution.

[0031] (2) Inject the newly obtained precursor NaHTe into the CdCl with thioglycolic acid (TGA) at a pH of 10.5 for nitrogen and deoxygenation 2 In aqueous solution, to which 228.34 mg of CdCl was added 2 2.5H 2 O and 138.5 μL of TGA. Mixed solution under nitrogen protection 100 oUnder the condition of C, the reflux reaction was performed for 9 hours and 72 hours to obtain the desired green fluorescent quantum dots and red fluorescent quantum dots.

[0032] (3) Mix 40 mL of the red fluorescent quantum dot stock solution obtained in step (2) and 80 mL of ethanol into the flas...

Embodiment 2

[0039] (1) 60.6 mg sodium borohydride (NaBH 4 ) and 51.04 mg of tellurium powder were added to the centrifuge tube, and then 3.0 mL of twice-distilled water was added to dissolve the solid completely; the centrifuge tube was placed in an ultrasonic machine for ultrasonic reaction, and the mouth of the tube was kept to vent gas, and the final white liquid was the desired The precursor NaHTe solution.

[0040] (2) Inject the newly obtained precursor NaHTe into the CdCl with thioglycolic acid (TGA) at a pH of 11.5 for nitrogen and deoxygenation 2 in aqueous solution to which 152.23 mg of CdCl was added 2 2.5H 2 O and 115.44 μL of TGA. The mixed solution was protected under nitrogen at 110 o Under the condition of C, the reflux reaction was performed for 9 hours and 72 hours to obtain the desired green fluorescent quantum dots and red fluorescent quantum dots.

[0041] (3) Mix 40 mL of the red fluorescent quantum dot stock solution obtained in step (2) and 100 mL of ethanol i...

Embodiment 3

[0048] (1) Add 45.4 mg sodium borohydride (NaBH 4 ) and 51.04 mg of tellurium powder were added to the centrifuge tube, and then 3.0 mL of twice-distilled water was added to dissolve the solid completely; the centrifuge tube was placed in an ultrasonic machine for ultrasonic reaction, and the mouth of the tube was kept to vent gas, and the final white liquid was the desired The precursor NaHTe solution.

[0049] (2) Inject the newly obtained precursor NaHTe into CdCl with thioglycolic acid (TGA) at a pH of 11.2 for nitrogen and deoxygenation 2 in aqueous solution to which 182.672 mg of CdCl was added 2 2.5H 2 O and 133 μL of TGA. Mixed solution under nitrogen protection 105 o Under the condition of C, the reflux reaction was performed for 9 hours and 72 hours to obtain the desired green fluorescent quantum dots and red fluorescent quantum dots.

[0050] (3) Mix 40 mL of the red fluorescent quantum dot stock solution obtained in step (2) and 96 mL of ethanol into the flask...

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Abstract

The invention relates to a dual-emission ratio-type quantum dot fluorescence probe for visual detection of aspirin, a preparation method and an application thereof and belongs to the technical field of preparation of material and detection of content of medicines. The preparation method of the probe includes following steps: preparing a precursor NaHTe solution from sodium borohydride, tellurium powder and water under an ultrasonic environment; adding the precursor to an aqueous solution of CdCl2.2.5H2O in the presence of thioglycollic acid; carrying out a reflux reaction with a nitrogen protecting condition to obtain required green fluorescence quantum dot and red fluorescence quantum dot; by means of a sol-gel method, wrapping the red fluorescence quantum dot with silicon spheres with amino group being connected; dispersing a green fluorescence quantum dot solution and the red fluorescence quantum dot wrapped with the silicon spheres in an MES buffer solution with addition of an EDC / NHS solution; and carrying out a reaction at room temperature in a dark place to obtain the dual-emission ratio-type quantum dot fluorescence probe. The dual-emission ratio-type quantum dot fluorescence probe is used in detection of the content of the aspirin through fluorescence quantitation and visualized analysis. The quantum dot fluorescence probe is quite good in optical performance and stability and has a capability of visualizedly detecting the aspirin.

Description

technical field [0001] The invention relates to a double-emission ratio quantum dot fluorescent probe and its preparation method and application, belonging to the technical field of material preparation and drug content detection. Background technique [0002] Aspirin (aspirin), also known as acetylsalicylic acid (acetylsalicylic acid), is the earliest, most widely used and most common antipyretic and analgesic antirheumatic drugs. It has various pharmacological effects such as antipyretic, analgesic, anti-inflammatory, anti-rheumatic and anti-platelet aggregation, etc. Arthritis and toothache etc. It has the characteristics of rapid drug effect, stable drug effect, and easy diagnosis and treatment of overdose. However, with the widespread use of aspirin, its adverse reactions are gradually increasing. The more common symptoms are nausea, vomiting, upper abdominal discomfort or pain, etc., so it is very important to monitor the amount of aspirin. At present, chromatograph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 卫潇戴江栋李洪吉周志平闫永胜
Owner JIANGSU UNIV
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