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Preparation method of corn oligopeptide powder

A technology of corn oligopeptide powder and corn gluten powder, which is applied in food science and other directions to achieve the effect of improving efficiency, yield and quality

Inactive Publication Date: 2014-12-10
江苏伟楼生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prior art only proceeds from one aspect to prepare high-purity corn oligopeptide powder

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A kind of preparation method of corn oligopeptide powder is characterized in that, taking corn gluten powder as raw material, its processing step comprises:

[0017] (1) Take 200g of corn gluten powder, add 3.5Kg, stir and mix evenly;

[0018] (2) Use Na 2 CO 3 The solution adjusts the feed liquid to pH 9, heats the feed liquid to 70°C, and keeps stirring for 40 minutes; centrifuges the feed liquid and retains the slag;

[0019] (3) Add 3.5Kg of water to the slag, stir evenly, heat to 70°C and keep stirring for 40 minutes; centrifuge the material and liquid, and keep the slag;

[0020] (4) Add 3.5Kg water to the slag, stir and mix evenly, add 1.2g of amylase or glucoamylase, heat the feed liquid to 60°C, and keep stirring for 50 minutes; centrifuge the feed liquid and keep the slag; Dilute the slag with water, heat to 60°C, keep stirring for 50 minutes, then centrifuge and keep the slag for use;

[0021] (5) use Na 2 CO 3 Adjust the pH of the feed solution to 9, h...

Embodiment 2

[0028] Take 2000g of corn gluten powder, add 2.5Kg, stir and mix evenly;

[0029] (2) Use Na 2 CO 3 Adjust the feed liquid to a pH of 8.5, heat the feed liquid to 60°C, and keep stirring for 50 minutes; centrifuge the feed liquid and retain the slag;

[0030] (3) Add 2.5Kg of water to the slag, stir evenly, heat to 60°C and keep stirring for 50 minutes; centrifuge the material and liquid, and keep the slag;

[0031] (4) Add 2.5Kg of water to the slag, stir and mix evenly, add 2g of amylase or glucoamylase, heat the feed liquid to 70°C, and keep stirring for 40 minutes; centrifuge the feed liquid and keep the slag; Dilute the material with water, heat to 70°C, keep stirring for 40 minutes, then centrifuge, and retain the slag;

[0032] (5) use Na 2 CO 3 Adjust the feed solution to a pH of 8, heat the feed solution to 75°C, then add 2g of alkaline protease, and enzymolyze it for 2 hours; inactivate the enzyme at high temperature; centrifuge the enzymatic hydrolyzate, and ke...

Embodiment 3

[0039] The corn oligopeptide powder that makes in embodiment one, two is analyzed and detected, and its crude protein content is 91.2%, and fat content is 1.2%, and molecular weight is respectively as follows:

[0040] Molecular weight range

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Abstract

The invention discloses a preparation method of corn oligopeptide powder, which belongs to the field of biotechnology. The preparation method comprises the following steps: by taking corn protein powder as a raw material, adding alkali and amylase or glucoamylase into the corn protein powder to process, so that effects of degreasing, and removing impurities such as starch are achieved so as to obtain high-purity corn protein powder, by taking the obtained high-purity corn protein powder as an enzymolysis substrate, hydrolyzing the enzymolysis substrate by using alkaline and neutral proteases, performing processes such as multistage separation and purification, concentration and spray drying on the obtained product, and finally, drying the obtained product into powder. The preparation method of corn oligopeptide powder disclosed by the invention has the characteristics of simple process and strong operability, and obviously improves the productive rate, and prepared corn oligopeptide powder is high in crude protein content and small in molecular weight, and can be widely applied to the development and production of foods and health products.

Description

technical field [0001] The invention relates to a preparation method, in particular to a preparation method of corn oligopeptide powder, and belongs to the field of biotechnology. Background technique [0002] Corn is one of the three major food crops in the world, and it is also a traditional crop in my country. With the continuous growth of corn production and the expansion and expansion of corn starch plants, its by-product - corn gluten meal (commonly known as yellow powder) has also increased significantly. [0003] Corn gluten meal is a by-product of producing starch or extracting ethanol from corn grains. Its protein content is 60% to 70%, and the rest is 20% of starch and about 10% of cellulose, vitamin A and other nutrients, and 15% A kind of inorganic salt and yellow pigment unique to corn. Because the amino acids in corn gluten meal are unbalanced, and the finished product has a special taste and color, it is mainly used in roughage in China, or it is directly d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/02A23L2/84A23L2/74
CPCA23J3/346
Inventor 孙伟楼刘凌冯之涛谢静莉
Owner 江苏伟楼生物科技有限公司
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