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Method for artificial cultivation of ganoderma mastoporum pat fruit body

A technology of artificial cultivation of ganoderma lucidum fruiting body, applied in the field of artificial cultivation of sessile purple ganoderma lucidum fruiting body, can solve problems such as failure to cultivate successfully, achieve the effect of meeting demand and alleviating ecological damage

Inactive Publication Date: 2014-12-10
GUIZHOU ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Folks are used as a substitute for Ganoderma lucidum, but it has not been successfully cultivated so far

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1): Separation of fresh field fruiting bodies of Sessile purple Ganoderma mastoporum (Lév) Pat or fruiting body tissues or spores obtained from cultivation, and then take 2 mm × 2 mm sterile tissue pieces and inoculate them on the slant PDA medium Cultivate for 25-35 days at 25-28°C and a relative humidity of 65%-75%, until the mycelia cover the slope, and then select vigorous, strong and uniform bacterial blocks (soybean size or 2mm×2mm volume inoculum) to inoculate Cultivate for 10-30 days at 25-28°C and relative humidity of 65%-75% in the comprehensive PDA medium on the slope, until the mycelia cover the slope, and obtain vigorous, strong and uniform purple ganoderma lucidum which can be used for production. species, which can be directly used for production or preserved at 4°C for subsequent use; the formula of the slant PDA medium is that each liter of medium is prepared by the following method: first cut 200g peeled potatoes into small pieces, add 1000ml of disti...

Embodiment 2

[0026] This embodiment is basically the same as Embodiment 1, except for the following differences:

[0027] 1. The formula of the slant comprehensive PDA medium is as follows: each liter contains 80g of potato, 10g of glucose, 10g of agar, 3g of potassium dihydrogen phosphate, 2g of magnesium sulfate and 10mg of VB, the balance is water, pH 6-8, the preparation method is as follows: Weigh the portion and content respectively, first cut the potatoes into small pieces and put them in the pot, add water and boil for 20-30 minutes until the potatoes are soft but not rotten, then filter them with gauze, take the filtered juice, add agar to melt, then add glucose, phosphoric acid Stir potassium dihydrogen and magnesium sulfate evenly, make up to 1000ml, pH 6-8, and sterilize;

[0028] 2. The original seed sawdust culture medium is: the dry material is based on the total mass fraction of 100%, including 38% of corncobs, 40% of sawdust, 20% of bran, 1% of glucose and 1% of gypsum pow...

Embodiment 3

[0032] This embodiment is basically the same as Embodiment 1, except for the following differences:

[0033] The original seed sawdust culture medium is: the dry material is based on the total mass fraction of 100%, including 32% of corncobs, 40% of sawdust, 20% of bran, 5% of glucose and 3% of gypsum powder. The content is mixed evenly to form a dry material, and then water is added to the dry material, and the dry material is mixed evenly so that the water content is 60%, the pH is 6.5-7, and the original seed sawdust culture medium is obtained.

[0034] In this example, the fruiting bodies of the sessile purple ganoderma were successfully obtained, which can be used as raw materials or research materials.

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PUM

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Abstract

The invention discloses a method for artificial cultivation of a ganoderma mastoporum pat fruit body. By means of the method, artificial cultivation of the ganoderma mastoporum pat fruit body is achieved, the average biological efficiency reaches 40%, namely 40 g of fresh ganoderma mastoporum pat fruit body can be obtained through every 100 g of cultivation compost, and the ganoderma mastoporum pat fruit body can be used as a raw material or a researching material. Ecological damage caused by excessive picking of wild ganoderma in the current market can be relieved, and the requirement for ganoderma of people is met.

Description

Technical field: [0001] The invention belongs to the field of microorganisms, and in particular relates to a method for artificially cultivating fruit bodies of sessile purple ganoderma lucidum. Background technique: [0002] Sessile purple ganoderma [Ganoderma mastoporum (Lév) Pat.] is distributed in Guangdong, Guangxi, Hainan, Yunnan, and Guizhou in China. The main features of wild Sessile Purple Ganoderma lucidum are: basidiocarp is annual, sessile or with stalk base, corky. Cap fan-shaped, nearly round or shell-shaped, diameter 4-5×6-7.5cm, thickness 3-10mm, surface purple dark brown to purple black, with dense concentric ring patterns, longitudinal wrinkles, smooth, paint-like Glossy; edges are complete and sharp; the flesh is uniformly brown, 3-5.5mm thick, with a black crust in between; the tubes are 3-7mm long, dark brown; the surface of the pores is initially light white, light brown to dark brown; tubes Mouth nearly round, 5-6 per mm. Sessile or with stalk base....

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
Inventor 邓春英
Owner GUIZHOU ACAD OF SCI
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