Extraction method of total puerarin flavonoids and method for improving production performance of broiler chicken and quality of chicken meat by adopting total puerarin flavonoids
A technology of Pueraria total flavonoids and extraction method, which is applied to medical preparations containing active ingredients, pharmaceutical formulas, plant raw materials, etc., and can solve problems such as drug residues and meat flavor decline
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Embodiment 1
[0017] A method for extracting total flavonoids of Pueraria radiata, the steps of which are:
[0018] (1) Take the kudzu root medicinal material, wash and cut into small pieces;
[0019] (2) Add a 30-80% ethanol solution that is 6-10 times the volume of the kudzu root medicinal material, soak for 0.5-2 hours, take out the impregnation solution, and extract each portion of the kudzu root medicinal material 1-3 times;
[0020] (3) After mixing the impregnating solution, put it on the macroporous adsorption resin column, first elute the impurities with water, then elute with 30-70% ethanol, and collect the ethanol eluate;
[0021] (4) Concentrate the collected ethanol eluent, after concentration, add ethanol to make the concentration of ethanol in the concentrated solution reach 70-80%, and then filter to remove the precipitate;
[0022] (5) Concentrate the filtrate, add n-butanol for extraction, recover n-butanol, concentrate and dry, and the yield of Pueraria flavonoids is 5-1...
Embodiment 2
[0025] The method for improving broiler production performance and chicken quality with total flavonoids of puerariae root is characterized in that the total flavonoids of puerariae root is added to the basal diet of broiler chickens.
[0026] (1) Select 275 1-day-old AA broilers with an initial weight of 45-50 g, and randomly divide them into 5 treatment groups, with 5 replicates in each group and 11 birds in each replicate (see Table 1). Before the test, the chicken house and surrounding environment and test equipment were thoroughly disinfected. The test chickens were kept in cages, and the temperature, humidity, light and natural ventilation were controlled during the test period. On the first and second days, the chicken house was illuminated 24h / d, and then 23h / d, that is, the lighting was stopped for 1h at night. Keep the house temperature at 32-34°C in the first week, and stop heating when the temperature reaches 24°C. Free access to food and water, immunization accor...
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