Cultivation method for promoting growth of transgenic tomatoes and accumulation of astaxanthin

Abstract

Provided is a cultivation method for promoting growth of transgenic tomatoes and accumulation of astaxanthin. The cultivation method comprises the steps of taking the astaxanthin transgenic tomatoes as materials, sowing seeds of the transgenic tomatoes, transplanting seedlings growing for two months after emergency of seedlings, covering the transgenic tomato seedlings with nylon nets respectively to perform shading processing, measuring light intensity through a quantum illumination meter, performing processing respectively according to four light intensity gradients including shading 75 percent of light, shading 50 percent of light, shading 30 percent of light and performing full illumination, performing photosynthesis measurement respectively at a flowering phase and a fruit phase, harvesting the transgenic tomatoes for three times when the transgenic tomatoes are ripe, and measuring contents of the astaxanthin in the transgenic tomatoes. In the planting process, watering is performed in routine to ensure a good moisture state, cooling processing is performed through fans and wet curtains, and deinsectization and disease prevention are performed at regular intervals. According to the cultivation method, the astaxanthin transgenic tomatoes serve as cell factories to produce the astaxanthin efficiently, photosynthetic capacity of the transgenic tomatoes and astaxanthin contents in fruits are optimal under the conditions that the light intensity is 30 percent of sunlight, and the foundation is laid for establishing an artificial efficient astaxanthin-rich transgenic tomato cultivation technique system.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a cultivation method for promoting the growth of transgenic tomato and the accumulation of astaxanthin. Background technique [0002] The carotenoids (such as β-carotene, lutein and zeaxanthin) that photosynthetic organisms participate in photosynthesis are non-ketone carotenoids, which are the only source of vitamin A synthesized by the human body. Some unicellular green algae can also synthesize and accumulate ketocarotenoids, such as astaxanthin, under stress. Astaxanthin's unique molecular structure and strong antioxidant activity endow it with high-efficiency anti-radiation, anti-aging, anti-tumor and prevention of cardiovascular and other diseases. This makes astaxanthin a new favorite in industries such as health and beauty. However, the sources of natural astaxanthin are very limited, and humans generally obtain astaxanthin from eating seafood such as shrimp, cr...

AI Technical Summary

Problems solved by technology

At present, how to promote the growth of astaxanthin transgenic tomato plants and the accumulation of astaxanthin content in fruits by adjusting the environmental conditions of facility cultivation is still a blank

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