A kind of creation method of switchgrass mutant

Abstract

The invention discloses a creation method of switchgrass mutants. The creation method comprises the following steps: after a lots of switchgrass multiple shoots are cultured in a tissue culture manner, stripping out individual multiple shoots, cutting off the upper sheaths after acclimatization under natural conditions, performing soaking treatment on the parts 1.5-2cm above the left base parts by use of a solution of ethylmethane sulfonate (EMS) for 3 hours, and after the completion of the soaking treatment, transplanting the multiple shoots of which the sheaths are cut off into a culture medium for culture, and after complete rooting, transplanting to a greenhouse or land, and screening out the mutants according to the phenotypes of the switchgrass. The method is capable of realizing large-scale processing of the mutational original acceptor material and thus increasing the basic number of the variants, and meanwhile, a near-isogenic line material only different from the original acceptor material in target traits can be obtained; and in addition, the mutagenesis process is simple and feasible, the processing cost is relatively low, and the breeding period can be effectively shortened; in short, the method is suitable for creating new materials for the modern biotechnology research of the switchgrass.

Description

technical field [0001] The invention belongs to the technical field of plant breeding and relates to a method for creating a switchgrass mutant. Background technique [0002] Switchgrass (English name Switchgrass, Latin name Panicum virgatum Linn) is a perennial grass C 4 The plant originates from the North American prairie, and has tall plants, simple cultivation, strong resistance to stress, and high annual biomass output. Originally, switchgrass was mainly used as feed crops and garden plants, and it was also used in soil erosion control and other aspects, showing excellent utilization prospects. At the end of the 20th century, the United States took the lead in selecting it as a model bioenergy plant. Since then, various countries have increased their research on the development of switchgrass. The use of modern biotechnology to genetically improve and develop switchgrass has become one of the research hotspots in the field of bioenergy at home and abroad. [0003] Cr...

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Problems solved by technology

However, so far there has been no report on the use of clones as mutagenic materials in the study of switchgrass

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