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Method for obtaining orange filial generation through embryo rescuing

An embryo rescue and citrus technology, which is applied in the field of citrus hybrid offspring obtained through embryo rescue, can solve problems such as hindering the development of zygotic embryos, abortion or degeneration of zygotic embryos, multiple embryos in citrus seeds, and long breeding cycles, so as to avoid abortion , Remarkable and operable effects

Inactive Publication Date: 2014-11-05
HUNAN AGRICULTURAL UNIV
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Problems solved by technology

The vigorous growth of the nucellus embryo often invades the embryo sac during the development of the zygotic embryo, hinders the development of the zygotic embryo, and causes the zygotic embryo to abort or degenerate in the early stage of development. Due to the polyembryony and long breeding cycle of citrus seeds, Citrus hybrid breeding has been difficult to succeed

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  • Method for obtaining orange filial generation through embryo rescuing
  • Method for obtaining orange filial generation through embryo rescuing

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Embodiment Construction

[0016] In order to make the purpose, technical solution and advantages of the present invention clearer, the present invention will be further described in detail through experiments below.

[0017] The female parent: Citrus sinensis Osbeck and the male parent: Citrus medica were used in the experiment, and they were planted in the National Citrus Germplasm Resource Garden.

[0018] A method for obtaining citrus hybrid offspring through embryo rescue provided by the present invention specifically comprises the following steps:

[0019] S01: Hybrid Pollination:

[0020] 1. Collect unopened citron petals, remove the petals with tweezers, remove the anthers and place them on a petri dish lined with paper, and then place the petri dish in an environment with a temperature of 22-25°C for 1-25°C. After 2 days, the anthers cracked and loosened, and the pollen was sieved to remove impurities, and the obtained citron pollen was packed in a dark bottle, sealed and stored in the refrige...

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Abstract

A method for obtaining an orange filial generation through embryo rescuing comprises the following steps: extracting plant pollens with strong disease resistance, carrying out hybrid pollination on female plants to obtain hybrid fruits, separating zygotic embryos and nucellar embryos, reserving the zygotic embryos, and culturing the zygotic embryos by an artificial culture medium. The method avoids the zygotic embryo abortion, can obtain anti-ulcer disease sexual progeny, and has the advantages of strong operability, simplicity, practicality, substantial effect and the like.

Description

【Technical field】 [0001] The invention relates to a method for obtaining hybrid offspring of citrus, in particular to a method for obtaining hybrid offspring of citrus through embryo rescue. 【Background technique】 [0002] Citrus has become the largest fruit in the world at present, with an annual total output of more than 100 million tons, accounting for about 25% of the world's total fruit output, and is the third largest traded agricultural product in the world. Citrus is produced in more than 100 countries and regions around the world. my country is one of the origin centers of citrus, and it is also a big producer of citrus, with the planting area and output ranking first in the world. Citrus bacterial cankerdisease (CBCD) is a worldwide disease and is an international and domestic plant quarantine target. The disease originated in Asia and is distributed in many countries and regions in the world. It is also called Asian disease. In recent years, the occurrence of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 陈仕贵陈扬朱丽娜张子烨崔勇
Owner HUNAN AGRICULTURAL UNIV
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