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Strong interference resistant homocysteine detection kit

A detection kit and anti-interference technology, applied in the field of homocysteine ​​detection kits, can solve the problems of expensive instruments, inaccurate detection results, unfavorable clinical detection applications, etc., so as to improve the analytical sensitivity and enhance the market. Competitiveness and the effect of being conducive to market promotion

Active Publication Date: 2014-10-22
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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AI Technical Summary

Problems solved by technology

Although the sensitivity and specificity are good, the instrument is expensive and cannot be popularized
High-performance liquid chromatography (HPLC) is a relatively mature and widely used method at present. The disadvantage is that there are many variations in sample processing, chromatographic conditions, sample detection and quantification, making it difficult to standardize
However, this detection method is easily interfered by impurities such as pyruvate in serum, resulting in inaccurate detection results. When compared with the chemiluminescence detection kit, the results are biased, which is not conducive to clinical detection applications.

Method used

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  • Strong interference resistant homocysteine detection kit
  • Strong interference resistant homocysteine detection kit
  • Strong interference resistant homocysteine detection kit

Examples

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Embodiment 1

[0031] S-adenosylmethionine (SAM) 0.1mM

[0032] NADH 0.3mM

[0033] Tris(2carboxyethyl)phosphine hydrochloride (TCEP) 5 mM

[0034] α-Ketoglutarate 5.0mM

[0035] Ascorbate oxidase 2.5 KU / L;

[0036] Reagent 2 (R2):

[0037] Hcy methyltransferase (HMTase) 5 KU / L

[0038] Glutamate dehydrogenase (GLDH) 10 KU / L

[0039] S-adenosyl homocysteine ​​(SAH) hydrolase (SAHase) 2.5KU / L

[0040] Adenosine deaminase (ADA) 5.0KU / L

[0041] Cystathionine β-synthase (CBS) 20-30 KU / L

[0042] The homocysteine ​​detection kit (enzyme method) described in this example, when in use, is measured by an automatic biochemical analyzer with dual reagent functions, such as Toshiba 40 automatic analyzer, and its operation is shown in Table 1:

[0043] Table 1 Homocysteine ​​detection reagent detection method

[0044]

[0045] Calculation: homocysteine ​​concentration = (determination / min÷standard / min)×C standard

[0046] Linear correlation experiment:

[0047]For the Sigma-Aldrich ultra...

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Abstract

The invention provides a strong interference resistant homocysteine detection kit. The kit comprises a component 1 and a component 2 according to a volume ratio of 4:1. The result obtained by using the kit is highly consistent with the result obtained through chemiluminescent detection, the accuracy and the sensitivity of detected clinic samples of the kit are better than that of routine reagents, and it is in favor of improving the clinic detection accuracy of homocysteine.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to a homocysteine ​​detection kit. Background technique [0002] Homocysteine ​​or homocysteine ​​is a variant of the amino acid cysteine, which contains an extra methylene group (-CH2-) before the thiol group (-SH) in the side chain. In 1969, McCully from The autopsy of children who died of hereditary homocystinuria found that there were extensive arterial thrombosis and pathological manifestations of atherosclerosis (AS) in the systemic circulation, thus proposing hyperhomocysteinemia (hyperhomocysteinemia, HHCY) can lead to the hypothesis of atherosclerotic vascular disease. Since then, scholars from various countries have done a lot of research on the relationship between HCY and cardiovascular and cerebrovascular diseases. Hcy is a thiol-containing amino acid, mainly derived from dietary methionine. It is an important intermediate product in the m...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6815G01N2333/90235
Inventor 甘宜梧岳庆妮李静谭柏清
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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