System and method for phase micro-interference imaging based on common-optical path system

Abstract

The invention discloses a system and a method for phase micro-interference imaging based on a common-optical path system. By taking full advantage of the stability of a coaxial system, a sample is put in a light beam which is expanded and collimated previously, object light and reference light share the same optical path, an angular-rotatable reflector changes the direction of a reference light beam so that the object light and the reference light beam are superposed, the angles of the reflector are changed to generate corresponding coaxial, off-axis and slight off-axis interference, and a phase interference pattern is microscopically amplified by a rear microscope and then a digital interference image can be generated on a CCD sensor. The spatial structural morphology of the phase object sample can be obtained by performing corresponding phase retrieval and deconstruction operation on the interference image. The system and the method have the advantages that noise generated in the measurement process is effectively measured in the use process, and the angles of the rotatable reflector are changed so that the included angle between the object light beam and the reference light beam is changed to adapt to the interference phase micro imaging of the phase object, including traditional coaxial interference, off-axis interference and slight off-axis interference; the system and the method are especially suitable for the fields of scientific research and clinical application of morphology detection and characteristic identification of biological cells.

Description

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AI Technical Summary

Benefits of technology

This technology improves upon existing systems by providing two main technical features: 1) ensuring reliable performance over time while reducing noise levels for future analysis purposes (the second one being important), 2) allowing for precise detection with minimal distortion from other sources such as light or electronics, 3) enabling effective utilization of space on both sides without losing resolution due to changes caused by external factors like temperature or humidity.

Problems solved by technology

Technologies described earlier include methods used to study cellular structures like blood vessels and nerves through their ability to see both brightly colored and invisible fluorescences when they pass through them. These technics involve studying how changes occur during fluid flow affect the shape of suspended particles within liquid crystal phases. By measuring the reflections of two different angles - either red or green – we aim to determine if any modifications made due to external factors cause significant variations in the resulting phase volumes obtained. To address issues related to achieving accurate measurement results without adding extra equipment, the patents propose developing new ways to measure phase interferometry while minimally disturbing surrounding areas.

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