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Tegillarca granosa antioxidant protein Peroxiredoxin-6

An anti-oxidation protein and anti-oxidant technology, which is applied in the direction of anti-toxins, biochemical equipment and methods, animal feed, etc., can solve the problems of degradation of disease resistance traits, decline of cockle populations, and increase of morbidity and mortality

Inactive Publication Date: 2014-10-22
ZHEJIANG WANLI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In recent years, due to the development of human activities in my country's coastal areas, the change and deterioration of the environment have led to the degradation of its disease resistance traits and the increase in mortality, resulting in a sharp decline in the population of cockles, a sharp drop in production, and a serious impact on economic benefits.

Method used

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  • Tegillarca granosa antioxidant protein Peroxiredoxin-6
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  • Tegillarca granosa antioxidant protein Peroxiredoxin-6

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Experimental program
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Embodiment Construction

[0013] The present invention will be described in detail below in conjunction with the examples.

[0014] 1. Separation of antioxidative protein Peroxiredoxin-6 (Prx6) from cockles

[0015] 1. All cockles used in this experiment were purchased from Ningbo market.

[0016] 2. Primers used in the experiment

[0017] The primers used in this experiment are detailed in Table 1:

[0018] Table 1: The cDNA clone of cockle Prx6 and the primers used in related experiments

[0019]

[0020] 3. Reagents used in the experiment

[0021] Isopropanol, ethanol, Trizol, RNase-Frees water, chloroform, TAE buffer, ampicillin, sodium citrate, X-gal, IPTG, glucose, SOB, agar powder and other common reagents were purchased from Shanghai Sangong; common DNA products The recovery kit and agarose gel kit were purchased from Tiangen Technology Co., Ltd.; the Advantage 2 Polymerase kit and the SMARTTM RNA cDNA amplification kit were purchased from Clontech; the Escherichia coli DH5α, rTag polyme...

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Abstract

The invention aims at providing a Tegillarca granosa antioxidant protein Peroxiredoxin-6 (Prx6) having an amino acid sequence of SEQ ID NO: 1. According to the invention, by virtue of screening and cloning the Tegillarca granosa antioxidant protein Prx6 gene, the full-length sequence of the Prx6 gene is obtained, and by virtue of bioinformatic analysis on the full-length sequence, the expression mechanism and control and regulation mechanism of the Prx6 gene are further explored and the contents of research on Tegillarca granosa immunology and molecular genetics are enriched. The invention provides a theoretical basis for subsequent research on the functional genes and molecular breeding and for discovery of candidate genes related to the major economic traits such as growth, reproduction, nutrition, metabolism and disease resistance.

Description

technical field [0001] The invention belongs to the technical field of functional gene screening, and in particular relates to Peroxiredoxin-6, an antioxidant protein of cockles. Background technique [0002] Antioxidant protein Peroxiredoxin is an antioxidant enzyme system belonging to the superfamily of antioxidant proteins. Its protein sequence and catalytic activity are different from other antioxidants. There are six members of the Peroxiredoxin family: Prx I, Prx II, Prx III, Prx IV, Prx V and Prx VI. Prx can protect the genome from the damage of reactive oxygen species and keep the genome intact by inhibiting cell senescence so as to prevent the occurrence of tumors. Therefore, Peroxiredoxin provides a new method for the treatment of tumors. The research on antioxidant proteins in plants mainly focuses on the role of plant growth and environmental adaptation. It not only acts as an antioxidant to reduce a variety of harmful peroxides, but also participates in signal...

Claims

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Application Information

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IPC IPC(8): C07K14/435C12N15/12A23K1/16A61P39/06A23K20/147
CPCC07K14/43509
Inventor 包永波林志华李成华
Owner ZHEJIANG WANLI UNIV
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