Method for separating cucurbitacin B from muskmelon vine by virtue of catalytic reaction

Abstract

The invention discloses a method for separating cucurbitacin B from muskmelon vines by virtue of catalytic reaction. The method comprises the following steps: an extraction process, namely taking 1 part of the muskmelon vines, soaking by using ethanol, performing reflux extraction, and filtering and concentrating an extracting solution to obtain an extract; the catalytic reaction, namely adding concentrated hydrochloric acid into the extract, then adding ferric trichloride hexahydrate, stirring and reacting, cooling to the room temperature, filtering, performing water rinsing, and drying; a refining process, namely stirring and dissolving a crude product by using ethyl acetate, then adding activated carbon, decoloring, concentrating to a small volume, and standing overnight to perform crystallization; a recrystallization process, namely dissolving a like fine product by using ethanol, filtering and concentrating to a small volume while hot, standing overnight to perform crystallization, and filtering; and putting the fine product in a vacuum drying box, and drying for 8 hours to obtain a cucurbitacin B fine product. The method disclosed by the invention is wide in raw material source and low in production cost, and can be used for reducing the damages of an acid environment to cucurbitacin B; the production rate reaches more than 60%, the purity is more than 99.0%, and the wastewater BOD (biochemical oxygen demand) is less than 100; the method is suitable for the industrial production of recycling water, and can be widely applied to plant extraction industries.

Description

technical field [0001] The invention relates to a method for separating cucurbitacin B by catalytic reaction, in particular to a method for separating cucurbitacin B from muskmelon vines by catalytic reaction. Background technique [0002] Cucurbitacin B is a new type of drug used in the treatment of acute and persistent chronic hepatitis and primary liver cancer. [0003] APIs are widely used in various fields such as pharmaceuticals, cosmetics and health products. [0004] According to literature reports, the content of cucurbitacin B in the melon pedicle is the highest, which can reach 0.6%---0.8%. Not high, and the limited quantity of product supply and other defects. [0005] Because of the novelty of the futures market and the strict confidentiality of the process route by domestic and foreign manufacturers, cucurbitacin B has not applied for many patents. [0006] The Chinese patent (201010235780.X) applied by Nanjing Zelang Agricultural Development Co., Ltd. on J...

AI Technical Summary

Problems solved by technology

[0014] 1. The source of raw materials: According to data calculations, 1 mu of melons can produce about 3-5 kilograms of dry melon stalks. In 2014, the price of melon stalks in the Bozhou market was 800 yuan / kg

And there is no way to provide tons of raw materials

[0015] 2. The temperature problem of enzymatic hydrolysis: Enzymatic hydrolysis is actually a fermentation process, which is greatly affected by temperature. There is a huge difference between winter and summer. As a product, the change of production season will lead to periodic changes in product delivery time. Very undesirable for an industrial product

[0016] 3. According to the technical information of the patent, the enzymatic hydrolysis time of 2 to 4 days greatly wastes the cost of manpower and material resources, and large-scale fermentation tanks and sites have many safety hazards and are not environmentally friendly

[0019] The biggest problem of this method is that simply using the conventional acid hydrolysis conversion method, only part of the cucurbitacin B-2-glucoside is converted, and the long-term acidic environment will lead to the destruction of cucurbitacin B, and the conversion yield is low. Significant waste of raw materials

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