Bee acute oral toxicity experimental apparatus
An oral toxicity and experimental device technology, applied in beehives, beekeeping, applications, etc., can solve problems such as population changes and individual death of bees, and achieve the effects of weak adsorption, labor saving, and convenient manufacturing and maintenance.
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Embodiment 1
[0026] Such as figure 1 , including beehive 1, nest foundation 2, two feeding ports 3a, 3b, honeybee introduction port 4, wire mesh groove 5, stainless steel wire mesh 6, conical pipe 7a, 7b and rubber stopper 8 for feeding, Wherein, the honeybee introduction port 4 and two feeding ports 3a, 3b are arranged on the top of the beehive 1, and the base 2 is arranged on the inner bottom surface and the two inner surfaces of the beehive, and the outer edges of the two sides near the front side are provided with concave holes. Groove 5, stainless steel wire mesh 6 is placed on the groove 5, can be drawn out and advanced from the groove flexibly.
[0027] Such as figure 2 Shown, nest foundation 2 is the wax board that is made by beeswax, and both sides are all neatly arranged with the room eye that shape is pentagon, for better implementing the present invention, described nest foundation area basically covers the inner bottom surface and the inner bottom surface of beehive. sides....
Embodiment 2
[0038] Embodiment 2: Honeybee reference substance experiment.
[0039] The bee acute oral toxicity test is used to determine the oral toxicity of the test substance to bees. Under the prescribed conditions, the bees are allowed to ingest different doses of the test substance medicinal honey through the oral route, and the dose of the test substance that causes 50% of the bees to die is determined. In this experiment, the acute oral toxicity of the reference substance dimethoate to bees was determined to determine whether the experimental device, experimental method and conditions meet the requirements.
[0040] 1 Materials and methods
[0041] 1.1 Test organisms
[0042] Italian honey bees (Apis mellifera L.), adult worker bees, were provided by Nanjing Jiangning United Beekeeping General Farm. During the experiment, healthy bees of the same size were selected and randomly assigned to each treatment or control group.
[0043] 1.2 Experimental conditions
[0044] Instrumen...
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