Preparation method of phosphatidyl serine

Abstract

The invention discloses a preparation method of phosphatidyl serine, which comprises the following steps: taking coarse soybean lecithin as a raw material, adding water-saturated butyl acetate, stirring, and performing reflux degradation; evaporating the butyl acetate under reduced pressure, adding methanol into the concentrate, and performing reflux dissolution; recovering the methanol from the methanol layer under reduced pressure, thus obtaining a methanol solvend; preparing the methanol solvend and organic solvent into a solution, then preparing the organic phase and an enzyme-containing water solution into a water phase, mixing the organic phase and the water phase, and reacting; after the reaction is finished, separating out the organic phase, washing the organic phase with water, and removing the organic solvent in vacuum; and dissolving the concentrate with anhydrous ethanol, stirring, separating out an anhydrous ethanol insoluble substance, and performing vacuum drying to obtain the phosphatidyl serine product. Compared with the prior art, the preparation method has the following advantages: (1) the used raw material is the coarse soybean lecithin, so that the phosphatidylcholine content is low, and the price is low; and (2) the whole process is simple and easy to industrialize, the content of the prepared phosphatidyl serine is 40% or above, and the purity is high. Thus, the preparation method disclosed by the invention has important meanings for phospholipid industry, health food and medicine industry in China.

Description

technical field [0001] The invention belongs to the technical field of phospholipids, and in particular relates to a preparation method of phosphatidylserine. Background technique [0002] Phosphatidylserine (PS), also known as serine phospholipid, diacylglyceryl phosphoserine, referred to as PS, is a kind of ubiquitous phospholipid, usually located in the inner layer of cell membrane, phosphoglycerides in phospholipid compounds, is the cell membrane One of the components related to a series of membrane functions. Especially in the nervous system of the human body, it is one of the important components of the cell membrane of the brain. At the same time, it plays an important role in regulating various functions of the brain (especially the memory and emotional stability of the brain). fluidity, permeability, and can activate the metabolism and synthesis of various enzymes. The existing synthetic methods include chemical synthesis and biological enzymatic synthesis, and th...

AI Technical Summary

Problems solved by technology

The chemical synthesis method has many side reactions, complex reactions, complex by-products, and very difficult separation, such as the method disclosed in CN103351403 patent literature

Biological enzymatic method has great advantages and many studies, such as US5965413, US6492146, US7049107, US5700668, US6878532 and other patent documents, but the disclosed enzymatic synthesis method is to synthesize phosphatidylcholine with relatively high purity phosphatidylcholine as raw material Serine (such as high-quality phospholipids with a phosphatidylcholine content of more than 50%), the product even exists in the form of calcium salt, the regeneration steps are cumbersome, and the production cost is high