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Analytical method and application of differentially expressed miRNAs in renal tissues of primary IGA nephropathy

A tissue-differentiated, primary technology, applied in the field of kidney disease research, can solve the problems of unknown pathogenesis, diverse clinical manifestations, and widely disparate prognosis, and achieve the effect of reasonable and feasible design.

Active Publication Date: 2016-08-24
戴勇 +1
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  • Application Information

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Problems solved by technology

So far, the pathogenesis is unknown, with racial and geographical differences, diverse clinical manifestations, lack of targeted treatment, and disparity in prognosis, which has caused great difficulties in early diagnosis and treatment

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  • Analytical method and application of differentially expressed miRNAs in renal tissues of primary IGA nephropathy
  • Analytical method and application of differentially expressed miRNAs in renal tissues of primary IGA nephropathy
  • Analytical method and application of differentially expressed miRNAs in renal tissues of primary IGA nephropathy

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Embodiment Construction

[0017] The analysis method and application of differential expression of miRNA in kidney tissue of primary IgA nephropathy will be further described in detail below mainly in conjunction with the accompanying drawings and specific examples.

[0018] The method for analyzing the differential expression of miRNA in kidney tissue of primary IgA nephropathy according to one embodiment comprises the following steps:

[0019] Step S110: Collect the total cellular RNA of the renal tissues of patients with primary IgA nephropathy and the normal group, separate and purify small RNA molecules with a length of 18-30 nt by 15% polyacrylamide gel electrophoresis, and separate the small RNA molecules by cDNA fragments are formed after reverse transcription, and adapter sequences are added to both ends of the formed cDNA fragments, and RNA libraries are formed after RT-PCR amplification.

[0020] Step S120: Sequencing the constructed RNA library, and then performing de-jointing and decontami...

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Abstract

The invention relates to an analysis method of differently expressed miRNA of kidney tissue of primary IgA nephropathy. Analytic processes of total RNA of kidney tissue of primary IgA nephropathic patients and total RNA of kidney tissue of a normal group are carried out through high-flux sequencing technology to obtain the miRNA of the kidney tissue of the primary IgA nephropathy. A further research in the differently expressed miRNA is beneficial to an explaination of the pathogenesis of the primary IgA nephropathy and provides a new approach for diagnosing and treating the primary IgA nephropathy. The analysis method of the differently expressed miRNA of the kidney tissue of the primary IgA nephropathy is reasonable and feasible in design, can effectively assist in establishing a map model of the differently expressed miRNA of the primary IgA nephropathy and can obtain relative information, as an intermediate result, of the primary IgA nephropathy.

Description

technical field [0001] The invention relates to the field of nephropathy research, in particular to an analysis method and application of miRNA differentially expressed in kidney tissue of primary IgA nephropathy. Background technique [0002] Primary IgA nephropathy (IgA nephropathy, IgAN) is a clinicopathological syndrome characterized by diffuse deposition of IgA-based immunoglobulin in the glomerular mesangium and capillary loops, with various clinical manifestations and pathological changes. IgAN is the most common primary glomerular disease in the world, and it occurs frequently in Asia, accounting for about 30% to 40% of primary glomerulonephritis in my country. It is also one of the main causes of end-stage renal disease. So far, the pathogenesis is unknown, with racial and geographical differences, diverse clinical manifestations, lack of targeted treatment, and disparity in prognosis, which has caused great difficulties in early diagnosis and treatment. Although t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12M1/00
CPCC12Q1/6869C12Q2535/122C12Q2525/207C12Q2537/165
Inventor 戴勇谭奎璧
Owner 戴勇
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