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Microecological feed additive and preparation method thereof

A micro-ecological feed and feed additive technology, which is applied in the molding or processing of animal feed, animal feed, animal feed, etc., can solve the problems of product quality that is difficult to meet standards, low content of effective metabolites, and poor control of various conditions. , to achieve the effect of good quality, reducing the degree of bacterial contamination and improving animal production performance

Active Publication Date: 2014-09-17
CHANGCHUN BORUI FEED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solid-state fermentation process is simple. Although liquid fermentation needs dehydration, collection, and drying procedures, the bacteria are easily infected by bacteria after multi-stage cultivation and open fermentation. The production time is long, the floor area is large, and the labor intensity is high. , various conditions are not well controlled, the content of effective metabolites is low, and the product quality is difficult to meet the standard, among which the contamination of bacteria is a common problem in solid fermentation production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A micro-ecological feed additive is obtained by compounding spray-dried yeast metabolites and plantaractobacillus metabolites in a mass ratio of 1:1.

[0040] A preparation method of micro-ecological feed additive, comprising the following steps

[0041] A, preparation of yeast metabolites: (1) Inoculate the preserved Saccharomyces cerevisiae strains into YPD slant medium, and culture them statically at 28~30°C for 48 hours to obtain revived strains; (2) Inoculate the revived yeast strains into liquid YPD (3) Inoculate the first-grade seed liquid into the screened aerobic fermentation medium in an amount of 4-10%, and ferment aerobically at 28-30°C for 24-48 hours to obtain the second-grade seed liquid (4) liquid anaerobic fermentation: inoculate the secondary seed liquid into the liquid anaerobic fermentation medium with an inoculum size of 20%, and anaerobic fermentation at 20° C. for 24 hours to obtain anaerobic fermentation products; (5) cell autolysis: papaya The ...

Embodiment 2

[0045] A micro-ecological feed additive is obtained by compounding spray-dried yeast metabolites and plantaractobacillus metabolites in a mass ratio of 1:2.

[0046] A preparation method of a micro-ecological feed additive, the preparation method comprises the following steps

[0047] A: Preparation of yeast metabolites: same as in Example 1

[0048] B: Preparation of lactic acid bacteria metabolites: same as Example 1

[0049] C: Preparation of complex metabolites: compound the spray-dried yeast metabolites and lactic acid bacteria metabolites in a mass ratio of 1:2.

Embodiment 3

[0051] A micro-ecological feed additive, which is composed of yeast metabolites and plant

[0052] The metabolites of Lactobacillus are compounded according to the mass ratio of 2:1.

[0053] A preparation method of a micro-ecological feed additive, the preparation method comprises the following steps

[0054] A: Preparation of yeast metabolites: same as in Example 1

[0055] B: Preparation of lactic acid bacteria metabolites: same as Example 1

[0056] C: Preparation of compound metabolites: the yeast metabolites and lactic acid bacteria metabolites were combined by spray drying

[0057] 2:1 mass ratio for compounding.

[0058] Product efficacy verification

[0059] 1. Broiler chicken feeding experiment program

[0060] 180 1-day-old AA broiler chickens were selected, and the test was randomly divided into 6 groups, with 3 repetitions in each group, and 10 chickens in each repetition. Dried different metabolite products were fed to broilers in an amount of 0.6% (mass ra...

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PUM

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Abstract

The invention relates to a microecological feed additive and a preparation method thereof. The feed additive is obtained by mixing yeast metabolites dried by a spray drying technology with intracellular substances and lactic acid bacteria metabolites according to a certain ratio. The microecological feed additive is characterized in that a specific stress-resistance metabolite group is produced by a single-strain high-density liquid industrial fermentation technology, and the intracellular substances are released by a high-efficiency cell wall breakage technology; by the adoption of a technology for compounding multiple single strain fermentation metabolites, a terminal achieves a product stacking effect, and the functionality of the product and the service range are expanded. The product has an important significance on promotion of animal growth, increasing of the feed utilization rate, prevention of diseases, enhancement of organ immunity and improvement of the environment.

Description

technical field [0001] 本发明属于微生物发酵领域,特别涉及一种微生态饲料添加剂及其制备方法。 Background technique [0002] 微生态制剂,是指在微生态学理论的指导下,调整微生态平衡失调,保持微生态平衡,提高宿主健康水平的正常菌群及其代谢产物和选择性促进宿主正常菌群生长的物质制剂总称。 [0003] 微生态饲料添加剂的主要作用为:调节动物肠道微生态平衡,提高动物生长率,提高饲料利用率,促进动物对钙、镁、铁的吸收;抑制肠道有害微生物的繁殖,提高动物健康水平,增强动物免疫力;无毒副作用,无耐药性,无病原性,被视为绿色饲料添加剂之一。 [0004] 微生态饲料添加的优势在于: [0005] 1、避免长期滥用抗生素带来的负面影响如药物残留、机体产生耐药菌株、动物机体免疫力下降、环境污染、食品的不安全性等。 [0006] 2、避免活菌制剂使用中存在的不易长期保存、不耐高温、活性随着储存时间逐渐降低、在动物肠道定植困难、通过胃肠道时抗灭活能力不强。 [0007] 酶制剂在使用过程中存在功效的单一、作用范围窄、功能性组分容易失活等弊端。 [0008] 酵母是一类单细胞低等真核生物,它即具有类似原核生物易培养、繁殖快、便于遗传操作等特性,又具有典型真核生物的分子和细胞生物学特性。酿酒酵母是人类利用最早的微生物,现已广泛应用与食品、饲料、医药、酿造工业等领域。 [0009] 酵母代谢物是在特定工艺条件控制下由优选的酿酒酵母在适宜的液体培养基中经过大量的繁殖,达到所需的数量级后,按不同的工艺条件接入液体厌氧发酵培养基中,在完全厌氧条件下,通过调控不同的发酵条件使其产生大量的对肠道有益菌增值有促进作用的代谢产物。其不同于常见的酵母产品,是由特定的酵母菌株在特殊的工艺条件控制下产生的对肠道有益菌有正向调节作用的复合代谢物组,其富含B族维生素、矿物质、消化酶、较齐全的氨基酸、小肽及未知生长因子,是集营养与保健为一体的饲料功能性添加剂,其作用效果不依赖于活酵母菌,保存期长,产品性能稳定。 [0010] 酵母代谢物一般的生产工艺主要是固态厌氧发酵法。固态发酵法工艺简单,虽然免去了液体发酵需脱水、收集、干燥等程序,但菌种经多级培养和开放式发酵易受杂菌感染,生产时间长,占地面积大,劳动强...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/00A23K10/10A23K10/18A23K40/00
Inventor 甄玉国赵小丽张学峰郑艳秋关艳玲赵巍
Owner CHANGCHUN BORUI FEED
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