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Method and model for predicting lodging resistance of soybeans

A lodging resistance and soybean technology, applied in the biological field, can solve problems such as large limitations, scientific evaluation of lodging resistance of difficult varieties, and environmental vulnerability, and achieve the effects of saving production costs, convenient and fast detection methods, and improving selection efficiency.

Inactive Publication Date: 2014-09-10
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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Problems solved by technology

However, this type of evaluation method is affected by climatic conditions (wind, rain, etc.), and it is difficult to scientifically evaluate the lodging resistance of varieties in case of climatic conditions that are not conducive to lodging.
Morphological traits related to lodging, such as plant height, stem diameter, shoot weight, crown-to-root ratio, etc., have also been reported as single indicators for soybean lodging resistance identification. Since there are few factors involved in a single indicator and limitations are large, it is used for The evaluation lacks accuracy, and the morphological traits of different varieties are different. When lodging does not occur, it is difficult to determine the difference in lodging resistance of different materials with a certain trait index
Many studies have attempted to find morphological indicators related to soybean lodging as indirect selection parameters for lodging resistance, such as plant height, number of branches, stem strength, stem thickness, root system, shoot weight, etc., but the close relationship between them There is no definite and reliable conclusion. In conventional breeding methods, lodging resistance cannot be identified until the mature stage, and it is easily affected by the environment, especially wind and rain. Field identification errors are large and selection efficiency is low.
[0004] Therefore, so far, there has not been an exact and reliable method for the prediction of soybean lodging resistance

Method used

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  • Method and model for predicting lodging resistance of soybeans

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, the method for predicting soybean lodging resistance

[0025] A soybean recombinant inbred line population containing 406 families was derived by crossing the soybean varieties Zhongdou 29 (lodging rate 17.8%) and Zhongdou 32 (lodging rate 62.5%) with significant differences in lodging (Zhou Xinan et al., Three- and four-pod variation of soybean recombinant inbred lines and its relationship with yield, Chinese Journal of Oil Crops, 2005, 27: 22-25).

[0026] In the mid-pod stage, the stalk strength (SS) was measured with a Japanese stalk strength tester (DIK-7401, Daiki 5011 & Moisture). According to the instructions for use, push the measuring device hard and slowly at a distance of 20cm from the ground to make the stem tilt and form an angle of 30 degrees with the ground, and record the measured value displayed on the measuring device at this time. In the middle of each test plot, 10 plants were randomly measured, and the average value was converted int...

Embodiment 2

[0031] Embodiment 2, utilize lodging resistance model to identify the lodging resistance of soybean breeding material

[0032] 1. Soybean materials to be identified

[0033] The F11 family of Zhongdou 29×Zhongdou 32 was obtained as follows: Zhongdou 29 and Zhongdou 32 were crossed, and the offspring of the hybrids were self-crossed continuously, and the F3 was planted in ramet rows, and each subsequent generation was randomly selected from each row. Select a single plant to derive the next generation. Five individual plants were randomly selected from the same family in the F7 generation, the seeds were mixed, and different families were planted separately to obtain a recombinant inbred line population consisting of 406 F11 lines. Among them, the hybridization method from F2 to F11 is self-crossing.

[0034] 2. Using the lodging resistance model to identify the lodging resistance of soybean breeding materials

[0035]From the 406 families in step 1, 30 F11 families (numbere...

Embodiment 3

[0049] Embodiment 3, utilize lodging resistance model to identify the lodging resistance of soybean germplasm resources

[0050] 1. Unidentified soybean germplasm resources

[0051] 30 soybean germplasm resources (see Table 3).

[0052] 2. Using the lodging resistance model to identify the lodging resistance of soybean germplasm resources

[0053] (1) Determination of lodging resistance and related traits by random block experiment

[0054] 30 soybean germplasm resources (numbered 31-60) were planted, and the experiment was designed in randomized blocks with three replicated blocks. 30 plots were set up for each repetition, and all experimental materials were planted in the experimental farm of the Institute of Oil Crops, Chinese Academy of Agricultural Sciences. Three rows are planted in each plot, the row length is 3.5m, the row spacing is 0.4m, and the plant spacing is 0.1m.

[0055] 1) Model predicts lodging resistance

[0056] Then randomly and continuously sample 5 ...

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Abstract

The invention discloses a method and a model for predicting the lodging resistance of soybeans. The method provided by the invention comprises the steps of (1) respectively measuring the numerical values of the following seven characters of the soybeans to be measured: stalk strength, plant height, stem diameter, branching number, root length, root weight and overground weight; (2) substituting the numerical values of the stalk strength, the plant height, the stem diameter, the branching number, the root length, the root weight and the overground weight into the lodging resistance model of the soybeans to calculate the lodging resistance: LR=174.162-6.618*BN-2.643*PH+15.165*RW+5.549*SD+0.108*SS+0.027*RL-0.483*SW, wherein if LR is more than or equal to 75, the soybeans to be measured are lodging-resistant soybeans or candidate lodging-resistant soybeans; if LR is less than 75, the soybeans to be measured are easy-lodging soybeans or candidate easy-lodging soybeans. The experiments prove that the method is convenient and fast, and is not influenced by environment.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for predicting soybean lodging resistance and a model thereof. Background technique [0002] Soybean is an important crop, which plays an important role in the development of my country's national economy, the composition of world food crops and the production of international oil crops. It has become the main source of edible and forage vegetable protein and vegetable oil in all countries in the world. Plant lodging is a common and serious problem in soybean production, and has become one of the main limiting factors to achieve high, stable, and high-quality soybean yields. Lodging is a phenomenon in which plant stems change from a natural upright state to a permanent dislocation caused by external factors such as wind and rain. The middle and late stages of soybean growth coincide with the rainy season in summer. The surface is wet and the soil is loose, which is easy to c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00
Inventor 陈海峰杨中路单志慧沙爱华邱德珍张婵娟陈李淼袁松丽张晓娟陈水莲万乔周蓉周新安
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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