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Super-long protein and polypeptide separation capillary liquid chromatography column system with automatic temperature control

A liquid chromatography column and separation capillary technology, which is applied to the polypeptide separation capillary liquid chromatography column system and the field of ultra-long proteins, can solve the problems such as the inapplicability of ultra-long capillaries and the inability to fix capillary columns well, and achieve sample separation. , Simple to make, the effect of reducing viscosity

Inactive Publication Date: 2014-09-10
EAST CHINA UNIV OF SCI & TECH
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The length of the currently used liquid chromatography column thermostat is generally less than 50cm, which is not suitable for ultra-long capillary tubes, and cannot fix the capillary column well

Method used

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  • Super-long protein and polypeptide separation capillary liquid chromatography column system with automatic temperature control
  • Super-long protein and polypeptide separation capillary liquid chromatography column system with automatic temperature control
  • Super-long protein and polypeptide separation capillary liquid chromatography column system with automatic temperature control

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Embodiment 1

[0023] Such as figure 1 , figure 2 , image 3 As shown, the self-temperature-controlled ultra-long protein and polypeptide separation capillary liquid chromatography column system consists of an ultra-long capillary liquid chromatography column (1), a capillary column support (2), a shell (3), and a temperature control module (4) , an insulation layer (5), a semiconductor thermoelectric cooling sheet (6), a thermocouple (7), a control panel (8) and an external power supply (9). The bracket (2) is fixed in the middle of the housing (3); the filled ultra-long capillary column (1) is coiled on the bracket (2), and the two ends of the column are respectively inserted into the small holes reserved on the edge of the housing (2). Stretch out; after the capillary is installed, fill the insulation material (5) between the capillary column (1) and the shell (2); the control panel (8) is fixed in the middle of the shell (2), and placed The thermocouple (7) inside and close to the co...

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Abstract

The invention relates to a super-long protein and polypeptide separation capillary liquid chromatography column system with automatic temperature control. A support frame is fixed at the intermediate part of a shell; a filled capillary liquid chromatography column is wound on the support frame, and both ends of the filled capillary liquid chromatography column respectively protrude out of a small hole reserved in the edge of the shell; after a capillary is arranged, a heat-insulating material is filled between the capillary column and the shell; a control panel is fixed at the intermediate part of the shell and connected with a thermocouple which is arranged in the ring of the capillary column and close to the capillary column, and a temperature control module fixed in the ring of the capillary column and a semiconductor thermoelectric refrigerating fin as a part of the shell of the column system are connected. The super-long protein and polypeptide separation capillary liquid chromatography column system disclosed by the invention is easy to manufacture and low in price, the column temperature can be increased by combining the super-long capillary column and a temperature control system, and the viscocity of a mobile phase can be effectively reduced, the back pressure can be reduced, the mass transfer rate between two phases can be accelerated and the sample separation can be conveniently and efficiently realized.

Description

【Technical field】 [0001] The invention relates to the technical field of chromatographic columns, in particular to an ultra-long protein and polypeptide separation capillary liquid chromatography column system with self-temperature control. 【Background technique】 [0002] Liquid chromatography is the most widely used analytical instrument in scientific research and industrial and agricultural production control. Utilizing higher pressure to provide high efficiency of liquid chromatography has always been its main means, but temperature has not received enough attention as an important parameter of liquid chromatography [document 1.Cuiru Zhu, DavidM.Goodall, Stephen A.C. Wren.. LCGC, 2005, 23(1): 54-72.]. Conventional liquid chromatography operates around room temperature. Since the mass transfer resistance of solute molecules in the liquid phase is much higher than that in the gas phase, the actual column length that can be used is limited by the back pressure, so the overa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/60
Inventor 张维冰刘海燕彭丽高羽张政
Owner EAST CHINA UNIV OF SCI & TECH
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