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Method for co-producing chondroitin sulfate and biological culture medium from porcine pancreas and chicken bones

A technology of chondroitin sulfate and porcine pancreas, which is applied in the biological field, can solve the problems of underutilized porcine pancreas and low utilization rate of pancreas, and achieve a high mineral content, full value utilization, comprehensive and sufficient nutrition Effect

Active Publication Date: 2014-09-10
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing extraction process mostly extracts a medicinal ingredient in the pancreas and discards a large amount of other active substances, so that the utilization rate of the pancreas is low, and the pancreas of the pig is not fully utilized.

Method used

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  • Method for co-producing chondroitin sulfate and biological culture medium from porcine pancreas and chicken bones
  • Method for co-producing chondroitin sulfate and biological culture medium from porcine pancreas and chicken bones
  • Method for co-producing chondroitin sulfate and biological culture medium from porcine pancreas and chicken bones

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specific Embodiment 1

[0058] Such as figure 1 shown, including the following steps:

[0059] Step 1, the chicken bone is crushed by a bone crusher, and the particle size after crushing is 2×2×2cm.

[0060] Step 2: Put the broken chicken bones into the extraction tank for variable temperature and hot-press extraction, pour water into the extraction tank so that the material-liquid ratio of the broken chicken bones to water is 1:2, and the extraction process is controlled by extracting Tank temperature and pressure to achieve variable temperature and hot-press extraction: when the temperature in the extraction tank reaches 95°C, keep it for 25 minutes, and the pressure is 0.25MPa; when it is 115°C, keep it for 40 minutes, and the pressure is 0.28MPa; MPa. After the extraction, the chicken aggregate liquid is discharged into the static tank.

[0061] Step 3: After the water in the standing tank is completely separated, the lower layer of feed liquid is discharged and passed through a 100-mesh vibra...

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Abstract

The invention discloses a method for co-producing chondroitin sulfate and a biological culture medium from porcine pancreas and chicken bones. By carrying out zymogen activation on porcine pancreas with a CaCl2 solution, the activities of the composite protease in porcine pancreas are greatly improved, and the expensive artificially synthetic trypsin can be completely replaced; the protein extractive solution prepared from chicken bones is subjected to sufficient hydrolysis reaction to obtain high-purity chondroitin sulfate of the purity is above 90%, and the yield is high. In addition, according to the method, broken chicken bones are extracted by a variable-temperature hot-pressing extraction method, chicken bones are more fully broken and dissolved by periodically changing the extraction temperature and pressure and thus nutrients, especially a plurality of minerals in chicken bones are fully dissolved, the contents of minerals in protein extracts are substantially increased, a biological culture medium with comprehensive nutrition is prepared from hydrolyzate and the full utilization of biological resources is achieved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for co-producing chondroitin sulfate and biological culture medium from pig pancreas and chicken bones. Background technique [0002] At present, China's total chicken output has increased from 1.39 million tons in 1984 to 13.7 million tons in 2012, ranking second in the world. Chicken skeleton is the main by-product of chicken processing, accounting for about 30% of the total weight of chicken. Chicken bone is quite nutritious, containing 16.3% protein, 14.5% fat, 3.95% calcium, 2.04% phosphorus and rich iron and other mineral elements , wherein the protein and fat content are similar to the same amount of fresh meat, while the mineral elements such as calcium, phosphorus and iron are several times that of fresh meat, and the ratio is appropriate. It can be seen that chicken bone has important comprehensive utilization and development value. However, for a long time due t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 张春晖李侠李海贾伟王金枝
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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