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Gene with immune effect

A technology of gene and gene encoding, applied in the field of CsghC1q gene, can solve the problems of unreported and less C1qDC protein

Active Publication Date: 2014-08-27
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

In fish, there are few reports on C1qDC protein research, and there are no reports on the cloning, expression changes, recombinant protein preparation and activity analysis of the complement molecule ghC1q

Method used

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Embodiment Construction

[0028] The method of the present invention will be further described below in conjunction with the accompanying drawings. However, the examples are for illustration only and are not limited thereto. For the experimental methods that do not indicate specific conditions in the following examples, conventional conditions can usually be used, such as the conditions described in the "Molecular Cloning Experiment Guide" written by J. Sambrook (Sambrook) etc., or according to the conditions suggested by the manufacturer run.

[0029] 1. Cloning and sequence of immune-related gene CsghC1q in half-smooth tongue sole

[0030] The partial cDNA sequence of CsghC1q gene of half-smooth tongue sole was obtained from the EST of the mixed immune tissue cDNA library of half-smooth tongue sole constructed by the Aquatic Genomics and Cell Engineering Laboratory of the Yellow Sea Research Institute of the Chinese Academy of Fishery Sciences. The long sequence is SEQIDNO:1( figure 1 ). And the ...

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Abstract

The invention provides a novel gene, which is separated from cynoglossus semilaevis and related to immune. The gene is subjected to in-vitro recombinant expression to obtain a recombinant expression product, and the antipathogen activities and functions of the recombinant expression product are identified to provide a gene resource and technical method for cynoglossus semilaevis disease control, green feed additives and antibacterial agents. The gene codes the following proteins: (a) protein with an amino acid sequence of SEQ ID NO:2; (b) protein including amino acids from the 117th-255th bits in (a) and has functions of (a); and (c) protein which is substituted, deleted or added by one or more amino acids in the amino acid sequence of (a) or (b) and has the protein activity in (a). The gene is closely related to immune resistance, the gene expression quantity of the gene is related to pathogenic stimulation, and the in-vitro recombinant protein of the gene can remarkably inhibit the proliferation of gram-negative bacteria, positive bacteria and viruses, so that the gene has application potentials in cynoglossus semilaevis disease control, green feed additive and antibacterial agents.

Description

technical field [0001] The invention belongs to the technical field of functional gene screening, and in particular relates to a novel gene related to immunity isolated from tongue sole, named CsghC1q gene. Background technique [0002] Tongue sole semismooth is an important marine fish cultured in the north and southeast coast of my country, and has high economic value. The annual output value of its artificial breeding has reached RMB 1.5-2 billion. However, in recent years, with the intensification of seawater pollution, the aquaculture environment around the land has continued to deteriorate, the scale of semi-smooth tongue sole farming has continued to expand, and the long-term use of fish species with certain excellent traits for multi-generation inbreeding has led to the emergence of semi-smooth tongue sole germplasm. Degeneration, decreased disease resistance. There are a large number of pathogenic bacteria of tongue sole in seawater, such as Vibrio anguillarum and...

Claims

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Application Information

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IPC IPC(8): C12N15/12C07K14/435C12N15/63A61K48/00A61K38/17A23K1/16A61P31/04A61P31/12A01N47/44A01P1/00A23K20/142A23K20/147A23K50/80
Inventor 沙珍霞陈亚东曾艳向晋松路飏陈松林公光业王天姿邵长伟
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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