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Long-chain dibasic acid production strain and its preparation method and application

A technology for producing long-chain dibasic acids and strains, which is applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., and can solve the problems of insignificant effect of long-chain dibasic acids, and achieve enhanced tolerance and fermentation The effect of shortening the cycle and increasing the conversion rate

Active Publication Date: 2017-02-08
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims to solve the technical problem that the effect of the existing strains on producing long-chain dibasic acids is not significant, and provides a high-efficiency long-chain dibasic acid-producing strain and its preparation method and application

Method used

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  • Long-chain dibasic acid production strain and its preparation method and application
  • Long-chain dibasic acid production strain and its preparation method and application
  • Long-chain dibasic acid production strain and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Metabolic Engineering Transformation of Bacterial Strains

[0036] 1) Genome sequencing

[0037] Genomic DNA of the long-chain dibasic acid-producing strain Candida was prepared using the Yeast DNAiso Kit of Bao Bioengineering Co., Ltd., and the specific operation was carried out according to the kit instructions. Genomic DNA is then subjected to steps such as library construction, next-generation sequencing technology sequencing analysis, and data assembly. Through genome annotation and data analysis, the gene information related to dibasic acid production of the production strain was obtained, and the coding sequences of genes related to the ω-oxidation and β-oxidation metabolic pathways of the strain were mined. For yeast, β-oxidation mainly occurs in the peroxisome, especially the β-oxidation of long-chain fatty acids, which only occurs in the organelle of peroxisome. In the ω-oxidative metabolic pathway, alkanes undergo a three-step enzymatic reaction ...

Embodiment 2

[0064] Embodiment 2: Fermentative production of long-chain dibasic acid

[0065] After the strains are cultured on a conventional slant, they are inserted into 50ml primary seeds and cultivated for 16 hours, and then the primary seeds are transferred to 500ml secondary seeds for 16 hours.

[0066] The formula of seed medium: yeast extract 1-8g / L, corn steep liquor 1-8g / L, sucrose 5-25g / L, KH 2 PO 4 4-12g / L, urea 0.5-4g / L, heavy wax 40-70g / L, sterilized at 121°C for 30 minutes. Among them, the sucrose and urea are separately sterilized at 110° C. for 20 minutes, and then combined and mixed evenly after sterilization.

[0067] After the secondary seed fermentation is completed, it is transferred into a 5L fermenter. Fermentation tank medium formula: yeast extract 1-8g / L, corn steep liquor 1-8g / L, sucrose 5-30g / L, KH 2 PO 4 4~15g / L, urea 0.5~4g / L, KNO 3 5~15g / L, NaCl 0.5~2.5g / L, sterilized at 121°C for 30 minutes. Among them, sucrose and urea are sterilized separately, ste...

Embodiment 3

[0072] According to the method of embodiment 2, just substrate is changed into methyl laurate from dodecyl straight-chain alkane.

[0073]

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Abstract

The invention relates to a long-chain dibasic acid producing strain and a preparation method and application thereof, and solves the problems of non-significant improvement effect of a long-chain dibasic acid strain in the prior art; the classification name is Candida (Candida sp.)TDTC016, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.8928. The long-chain dibasic acid producing strain can be widely used in the field of preparation of a long chain dibasic acid.

Description

technical field [0001] The invention relates to a bacterial strain and its preparation method and application, in particular to a long-chain dibasic acid production strain and its preparation method and application. Background technique [0002] Long-chain dibasic acid is an important chemical raw material with a wide range of uses, and can synthesize a series of high value-added chemicals such as spices, special nylon, and high-grade lubricating oil. Long-chain dibasic acids can be used in military fields, coatings of aerospace vehicles, pipelines, surface coatings and oil pipes of automobiles, etc.; A number of high-tech industries can develop more downstream industries and form a new industrial chain. [0003] In the past, long-chain dibasic acids were produced by chemical synthesis, and its patented technology was owned by foreign countries. The production of long-chain dibasic acids by chemical synthesis not only has a single product category, but also has complex syn...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/19C12P7/64C12P7/44C12R1/72
Inventor 赖小勤晏礼明杨勇葛书华陈远童陶勇傅深展
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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