A liver cancer-targeted multi-walled carbon nanotube drug-loaded composite material and its preparation method

A technology of multi-walled carbon nanotubes and composite materials, applied in the field of multi-walled carbon nanotube drug-carrying composite materials and their preparation, can solve the problems of difficulty in dispersion and easy accumulation of carbon nanotubes, and achieves high release rate and mild experimental conditions. , the effect of simple operation

Inactive Publication Date: 2016-02-17
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the characteristics of carbon nanotubes that are easy to accumulate and difficult to disperse, the research on carbon nanotubes needs to be further explored.

Method used

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  • A liver cancer-targeted multi-walled carbon nanotube drug-loaded composite material and its preparation method
  • A liver cancer-targeted multi-walled carbon nanotube drug-loaded composite material and its preparation method
  • A liver cancer-targeted multi-walled carbon nanotube drug-loaded composite material and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Dissolve 5mg of carbon nanotube composite material in 5ml of water, add 5ml of DOX (5mg) aqueous solution drop by drop while stirring, adjust pH to 9.0 with 0.1M sodium hydroxide solution, at room temperature Stir for 4h. Finally, the carbon nanotube solution containing doxorubicin was put into a dialysis bag (MW=8,000-14,000), the unconnected doxorubicin was removed by dialysis, and the solution was dialyzed with PBS buffer 3 times, 2L each time. Finally, the product is freeze-dried to obtain CNT / DOX.

[0041] (2) Weigh 2mg of doxorubicin, prepare 0.002M, 0.004M, 0.008M, 0.016M, 0.032M PBS solution (pH=7.4) and acetic acid solution (pH=5.8) respectively, and apply UV to the doxorubicin solution For detection, the absorption wavelength was set to 481nm, and the data were collected and fitted to the standard curve of adriamycin under two pH conditions.

[0042] (3) Weigh 2mg of drug-loaded complex in 2ml phosphate buffer (pH=7.4), fully dissolve it, put it into two dial...

Embodiment 2

[0045] (1) Dissolve 10.3 mg of carbon nanotube composite material in 10.3 ml of water, add 13 mL of DOX (6 mg) aqueous solution dropwise with stirring, adjust pH to 9.0 with 0.1 M sodium hydroxide solution, Stir at room temperature for 4h. Finally, the carbon nanotube solution containing doxorubicin was put into a dialysis bag (MW=8,000-14,000), the unconnected doxorubicin was removed by dialysis, and the solution was dialyzed with PBS buffer 3 times, 2L each time. Finally, the product is freeze-dried to obtain CNT / DOX.

[0046] (2) Weigh 2mg of doxorubicin, prepare 0.0025M, 0.005M, 0.01M, 0.02M, 0.04M PBS solution (pH=7.4) and acetic acid solution (pH=5.8) respectively, and apply UV to the doxorubicin solution For detection, the absorption wavelength was set to 481nm, and the data were collected and fitted to the standard curve of adriamycin under two pH conditions.

[0047] (3) Weigh 4.5 mg of the drug-loaded complex in 4.5 ml of phosphate buffer (pH = 7.4), fully dissolve it, ...

Embodiment 3

[0050] (1) Dissolve 10 mg of carbon nanotube composite material in 10 ml of water, add 5 mL of DOX (10 mg)-containing aqueous solution dropwise with stirring, and adjust the pH to 5.5, 6.5, and pH respectively with 0.1 M sodium hydroxide solution. 7.5, 8.5 and 9.5, stir at room temperature for 4h. Finally, put the carbon nanotube solution containing doxorubicin at different pH values ​​into a dialysis bag (MW=8,000~14,000), remove the unconnected doxorubicin by dialysis, and dialyzed 3 times with PBS buffer, each Times 2L. Finally, the product is freeze-dried to obtain CNT / DOX.

[0051] (2) Weigh 2mg of doxorubicin, prepare 0.001M, 0.002M, 0.004M, 0.012M, 0.036M PBS solution (pH=7.4) and acetic acid solution (pH=5.8) respectively, and perform ultraviolet treatment on the doxorubicin solution. For detection, the absorption wavelength was set to 481nm, and the data were collected and fitted to the standard curve of adriamycin under two pH conditions.

[0052] (3) Weigh 0.5mg of ea...

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Abstract

The invention relates to a liver cancer-targeted multi-walled carbon nanotube drug-loaded composite material and a preparation method thereof. The composite material is a compound of doxorubicin (doxorubicin) and a lactobionic acid modified multi-walled carbon nanotube CNT-PEI-FI-PEG-LA. The preparation method comprises the following steps: synthesizing the multi-walled carbon nanotube composite material CNT-PEI-FI-PEG-LA; formulating doxorubicin standard curves in two types of pH value environments; and dissolving the multi-walled carbon nanotube composite material in water, adding a doxorubicin hydrochloride aqueous solution, adjusting the pH value to neutrality and alkalinity, stirring for 4 hours, dialyzing, and carrying out freeze drying, thus obtaining the liver cancer-targeted multi-walled carbon nanotube drug-loaded composite material. The liver cancer-targeted multi-walled carbon nanotube drug-loaded composite material has the characteristics of pH sensitive release and liver cancer cell targeting, can be used for anti-tumor research and has very high practical value.

Description

Technical field [0001] The invention belongs to the field of liver cancer targeting nanomaterials and preparation thereof, and particularly relates to a liver cancer targeting multi-wall carbon nanotube drug-carrying composite material and a preparation method thereof. Background technique [0002] Carbon nanotubes can be roughly classified into single-walled carbon nanotubes (SWCNT) and multi-walled carbon nanotubes (MWCNT). They are graphite tubular crystals formed by curling single-layer or multilayer graphite according to a certain helix angle. According to the configuration, they can be divided into zig-zag, amchair and chiral. The tips of carbon nanotubes are mostly composed of pentagonal or heptagonal carbon rings. The shapes of carbon nanotubes are diverse, such as cylindrical, ring, coil and bamboo. Due to its unique properties, carbon nanotubes have shown good application prospects in biology, chemistry, and materials. However, because carbon nanotubes are easy to acc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/48A61K47/04A61K47/26A61K31/704A61P35/00
Inventor 朱利民陶磊
Owner DONGHUA UNIV
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