Gene transfer chip and gene transfer method
A gene introduction and chip technology, applied in the field of cell biology, can solve the problems of low accuracy and difficult continuous control of microbubbles.
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[0103] Such as Figure 4 Shown is a diagram of an experimental device based on a gene transfer chip in an embodiment, and the required equipment includes: a signal generator, a power amplifier, a microscope, a CCD, a gene transfer chip, a computer, and the like.
[0104] A signal generator is used to generate a radio frequency signal, and after being amplified by an amplifier, it is connected to a gene introduction chip. The continuous sinusoidal signal with low amplitude is used to move the microbubbles, and the instantaneous high-voltage pulse is used to break the microbubbles to perforate the cells and achieve the purpose of gene transfection.
[0105] Figure 5a ~ Figure 5c A process for precisely moving microvesicles to the area where the cells are located is described. DII lipophilic dye is combined with microbubble phospholipid membrane to produce red fluorescence, and DAPI dye is combined with cell nucleus to emit blue fluorescence, such as Figure 5ashown. Applyin...
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