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Gene transfer chip and gene transfer method

A gene introduction and chip technology, applied in the field of cell biology, can solve the problems of low accuracy and difficult continuous control of microbubbles.

Active Publication Date: 2016-05-18
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Prentice et al. used optical tweezers to achieve spatial manipulation of microbubbles. However, due to the balance between the focus range and focus intensity of optical tweezers, it is difficult to achieve large-scale continuous manipulation of microbubbles [P.Prentice, A. Cuschieri, K. Dholakia, M. Prausnitz, and P. Campbell, "Membraned disruption by optically controlled microbubble cavitation," Nat. Phys., vol.1, pp.107-110, 2005.]
[0007] The traditional sonoporation method is to study the quorum effect of microbubbles and cells, and the accuracy is low

Method used

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  • Gene transfer chip and gene transfer method

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Embodiment 1

[0103] Such as Figure 4 Shown is a diagram of an experimental device based on a gene transfer chip in an embodiment, and the required equipment includes: a signal generator, a power amplifier, a microscope, a CCD, a gene transfer chip, a computer, and the like.

[0104] A signal generator is used to generate a radio frequency signal, and after being amplified by an amplifier, it is connected to a gene introduction chip. The continuous sinusoidal signal with low amplitude is used to move the microbubbles, and the instantaneous high-voltage pulse is used to break the microbubbles to perforate the cells and achieve the purpose of gene transfection.

[0105] Figure 5a ~ Figure 5c A process for precisely moving microvesicles to the area where the cells are located is described. DII lipophilic dye is combined with microbubble phospholipid membrane to produce red fluorescence, and DAPI dye is combined with cell nucleus to emit blue fluorescence, such as Figure 5ashown. Applyin...

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Abstract

The invention discloses a gene introduction chip and a gene introduction method. The gene introduction chip comprises a piezoelectric substrate, a plurality of interdigital transducers, which are periodically arranged on the periphery of the piezoelectric substrate, and a micro canal arranged in the central part of the piezoelectric substrate; wherein the micro canal comprises a mono-disperse micro bubble preparation area and a cell culture area, the mono-disperse micro bubble preparation area is used to prepare mono-disperse micro bubbles by utilizing a flow focusing theory, and each mono-disperse micro bubble comprises a liposome membrane and gas contained in the liposome membrane. The foreign gene needed to be introduced into the chip can be mixed with the mono-disperse micro bubbles or can be embedded into the liposome membrane layer of the mono-disperse micro bubbles. The plurality of periodically-arranged interdigital transducers can simultaneously apply independent radio-frequency signals so as to form a two-dimensional flat stationary wave field in the micro canal, and thus the mono-disperse micro bubbles are captured and trapped in the potential wells of the stationary wave through a stationary wave field effect. Through adjusting the phase position of an input signal, people can change the potential well positions in the stationary wave field so as to move the mono-disperse micro bubbles.

Description

technical field [0001] The invention relates to the field of cell biology, in particular to a gene introduction chip and a gene introduction method. Background technique [0002] For a long time, humans have devoted a lot of energy to such diseases as malignant tumors, genetic diseases (such as hemophilia, cystic fibrosis, familial hypercholesterolemia, etc.) and infectious diseases (such as AIDS, rheumatoid, etc.), However, ideal results were not achieved. The occurrence of the above-mentioned diseases is closely related to the variation or abnormal expression of genes in the human body, so the most ideal cure method should be to correct them at the gene level. The introduction of gene therapy theory and continuous in-depth research have brought human beings into a new stage of development in the study of completely overcoming this type of disease, and have made great progress. Gene therapy refers to the introduction of normal genes or therapeutic genes into human target ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/42C12N15/09
CPCC12M23/16C12M35/04C12M35/08C12M41/04C12N15/88
Inventor 郑海荣孟龙蔡飞燕牛丽丽李飞肖杨
Owner SHENZHEN INST OF ADVANCED TECH
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