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Application method of long no-coding RNA (Lnc RNA (Ribonucleic Acid)) RMST (Rhabdomyosarcoma 2 associated Transcript)

A long-chain non-coding and application method technology, which is applied in the application field of preparing prognostic reagents for glioma patients, can solve the problem of inability to adapt to the prognosis judgment of human gliomas, poor prognosis of malignant gliomas in glioma patients, no specificity Sexual indicators and other issues, to achieve far-reaching clinical significance and promotion effect

Active Publication Date: 2014-08-06
CENT SOUTH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although some treatment methods including surgery, radiotherapy and chemotherapy have made great progress, the prognosis of patients with glioma, especially malignant glioma, is still poor.
At present, there is no reference standard for the prognosis of glioma, and there is no specific index, which is far from meeting the needs of the prognosis of human glioma

Method used

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  • Application method of long no-coding RNA (Lnc RNA (Ribonucleic Acid)) RMST (Rhabdomyosarcoma 2 associated Transcript)
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  • Application method of long no-coding RNA (Lnc RNA (Ribonucleic Acid)) RMST (Rhabdomyosarcoma 2 associated Transcript)

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Example 1 Preparation of LncRNA RMST kit for the prognosis of glioma patients (50 reactions)

[0025] 1. RNA stabilization solution 50ml

[0026] 2. Isopropanol 100ml

[0027] 3. Chloroform 100ml

[0028] 4. Trizol 50ml

[0029] 5. Enzyme-free water 10ml

[0030] 6.1μM random reverse transcription primer 50ul

[0031] 7.5× reverse transcription buffer 200ml

[0032] 8.10mM base triphosphate deoxyribonucleotides 100ul

[0033] 9.40U / μl RNase inhibitor 500ul

[0034] 10.200U / μl MMLV reverse transcriptase 50ul

[0035] 11. Premix Ex Taq 50ul

[0036] 12.10μM Lnc RNA RMST specific primer 30ul

[0037] Forward primer 5′-AACTCCGTGTCCCTTGTG-3′

[0038] Reverse primer 5′-GGCAGTGGGTGACTGATC-3′

[0039] 13. 10μM U6snRNA specific primer 30ul

[0040] The forward primer is 5′-ATTGGAACGATACAGAGAAGATT-3′

[0041] The reverse primer was 5'-GGAACGCTTCACGAATTTG-3'.

Embodiment 2

[0042] Example 2 Detection of Lnc RNA RMST in Glioma Tissue

[0043] 1. Preservation of glioma tissue: collect the glioma tissue to be tested and store it in a cryopreservation tube filled with RNA stabilization solution, and put it in a -80°C refrigerator for later use.

[0044]2. Extraction of RNA in tissues: Take an appropriate amount of specimen, add liquid nitrogen to the mortar after baking at 180°C for 6-8 hours, grind the specimen, grind to powder, add 1ml Trizol mortar specimen to the mortar, and grind into After liquid, transfer to tube, add 200μl / ml Trizol in chloroform, shake by hand for 15-30s, place on ice for 5min, centrifuge at 12000g at 4°C for 15min; carefully take the upper aqueous phase into a new tube, add pre-cooled Mix with isopropanol 0.5ml / ml Trizol, put it in a refrigerator at -20°C for 20min, centrifuge at 12000g at 4°C for 10min; discard the supernatant, add 1-2ml of ethanol diluted with 75% DEPC water, mix well, centrifuge at 7500g at 4°C for 5min,...

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Abstract

The invention discloses an application method of long no-coding RNA (Lnc RNA (Ribonucleic Acid)) RMST (Rhabdomyosarcoma 2 associated Transcript). The application method is used for preparing a prognosis preparation for glioma patients and particularly used for preparing a kit which is used for predicting the prognosis of the glioma patients by a real-time fluorescent quantitative analysis method. Confirmed by researches, the expression of Lnc RNA RMST in glioma tissue is increased, and the prognosis of the glioma patients with high Lnc RNA RMST expression is poorer than that of the glioma patients with low Lnc RNA RMST expression, so that by applying the expression of Lnc RNA RMST to the prognosis prediction of the glioma patients, a powerful basis for molecular biology can be provided for the prognosis of the glioma patients; the application method has far-reaching clinical significance and generalization performance.

Description

technical field [0001] The invention belongs to the field of tumor molecular biology, and in particular relates to an application method of LncRNA RMST in preparing prognostic reagents for glioma patients. Background technique [0002] Glioma originates from the ectoderm, accounting for about 40.49% of nervous system tumors. It is one of the most common tumors and can occur in people of any age. Invasion and metastasis occur. Although its incidence accounts for only 2% of adult tumors, its case fatality rate accounts for 10% of all cancer-related deaths. Although some treatment methods including surgery, radiotherapy and chemotherapy have made great progress, the prognosis of patients with glioma, especially malignant glioma, is still poor. At present, there is no reference standard for the prognosis of glioma, and there is no specific index, which is far from meeting the needs of the prognosis of human glioma. Therefore, judging the prognosis of human glioma in order to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 武明花刘长红余志斌徐刚李桂源
Owner CENT SOUTH UNIV
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