Testosterone detection reagent based on microparticle chemiluminescence immunoassay technology

Abstract

The invention discloses a testosterone detection reagent based on a microparticle chemiluminescence immunoassay technology; the testosterone detection reagent comprises the following components: 0.002%-0.01% of paramagnetic microspheres, 0.4 mug / ml-1 mug / ml of acridinium ester labeled testosterone antibodies, a composite testosterone release agent, a luminescence liquid A, a luminescence liquid B, 0ng / dl-1500ng / dl of a testosterone calibrator and a cleaning fluid with a certain concentration; and the testosterone release agent is a mixture of dihydrotestosterone, danazol and heparin sodium. The testosterone detection reagent has the advantages of simple operation, high sensitivity, fast detection efficiency, low cost, easy automation and the like, and by combination of the selected composite release agent and different binding globulin, combined-state testosterone in serum can be fully released, so that the content detection of total testosterone is more accurate.

Description

technical field [0001] The invention relates to the technical field of immunoassay, in particular to a testosterone detection reagent based on microparticle chemiluminescence immunoassay technology. Background technique [0002] Testosterone is a steroid hormone that is widely present in humans and animals. It can accurately detect its content in the body and judge its reproductive performance, normal physiological index and pathological concentration for disease diagnosis and treatment, family planning, and animal reproduction. It plays an important role in breeding and so on. Testosterone in men is produced by Leydig cells, and the testosterone content in men's serum is the most important parameter for confirming suspected testicular dysfunction, proving androgen deficiency and detecting testosterone replacement therapy. In women, testosterone is mainly synthesized in the ovaries, but also in the adrenal cortex, and the testosterone content in the female serum is used to ...

AI Technical Summary

Problems solved by technology

Due to the pollution of radioactive elements to the environment and its short half-life, etc., the operation and use are inconvenient, which limits the application of radioimmunoassay; and the enzyme immunoassay has defects such as low sensitivity and narrow detection range, which cannot meet clinical requirements.

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