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Bacillus coagulans and application thereof in calcium lactate production by in-situ product separating and fermentation

A technology for the separation of Bacillus coagulans and in-situ products, applied in fermentation, microbial-based methods, bacteria, etc., can solve the problems of substrate loss, microbial cytotoxicity, and high equipment requirements, and achieve cost savings, low nutritional requirements, and low cost cheap effect

Active Publication Date: 2014-07-30
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common in-situ product separation and fermentation generally adopt product separation technologies such as electrodialysis, ion exchange, and organic solvent extraction. Some of these separation technologies require high equipment, some are cytotoxic to the microorganisms used in fermentation, and some will cause a large Substrate loss, there are certain limitations in the application of industrial production

Method used

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  • Bacillus coagulans and application thereof in calcium lactate production by in-situ product separating and fermentation
  • Bacillus coagulans and application thereof in calcium lactate production by in-situ product separating and fermentation
  • Bacillus coagulans and application thereof in calcium lactate production by in-situ product separating and fermentation

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Experimental program
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Effect test

Embodiment 1

[0041] The composition of medium used in the present embodiment is as follows:

[0042] Slant medium: glucose 10g / L, yeast powder 10g / L, soybean peptone 5g / L, calcium carbonate 5g / L, agar powder 15g / L, pH 6.0.

[0043] Seed medium: glucose 80g / L, yeast powder 10g / L, soybean peptone 5g / L, pH 6.0.

[0044] Fermentation medium: glucose 200g / L, yeast powder 12.6g / L, soybean peptone 1.2g / L, cottonseed protein 3g / L, sodium nitrate 1g / L, ammonium chloride 1g / L, pH 6.2.

[0045] The steps of producing optically pure L-calcium lactate by the open in-situ product separation and fermentation method of the present invention are as follows:

[0046] (1) Slant culture: inoculate Bacillus coagulans H-1 on the slant medium, and culture at 50-60°C for 24-48 hours.

[0047] (2) Seed culture: inoculate the slant culture of step (1) into 30 mL seed medium under sterile conditions, add 40 g / L calcium carbonate to control the pH of the fermentation broth, and culture at 50-60 °C for 10 ~ 24 hour...

Embodiment 2

[0054] The composition of medium used in the present embodiment is as follows:

[0055] Slant medium: glucose 10g / L, yeast powder 10g / L, soybean peptone 5g / L, calcium carbonate 5g / L, agar powder 15g / L, pH 6.0.

[0056] Seed medium: glucose 80g / L, yeast powder 10g / L, soybean peptone 5g / L, pH 6.0.

[0057] Fermentation medium: glucose 200g / L, yeast powder 12.6g / L, soybean peptone 1.2g / L, cottonseed protein 3g / L, sodium nitrate 1g / L, ammonium chloride 1g / L, pH 6.2.

[0058] The steps of producing optically pure L-calcium lactate by the open in-situ product separation and fermentation method of the present invention are as follows:

[0059] (1) Slant culture: inoculate Bacillus coagulans H-1 on the slant medium, and culture at 50-60°C for 24-48 hours.

[0060] (2) Seed culture: inoculate the slant culture of step (1) into 30 mL seed medium under sterile conditions, add 40 g / L calcium carbonate to control the pH of the fermentation broth, and culture at 50-60 °C for 10 ~ 24 hour...

Embodiment 3

[0067] The composition of medium used in the present embodiment is as follows:

[0068]Slant medium: glucose 10g / L, yeast powder 10g / L, soybean peptone 5g / L, calcium carbonate 5g / L, agar powder 15g / L, pH 6.0.

[0069] Seed medium: glucose 80g / L, yeast powder 10g / L, soybean peptone 5g / L, pH 6.0.

[0070] Fermentation medium: glucose 200g / L, yeast powder 12.6g / L, soybean peptone 1.2g / L, cottonseed protein 3g / L, sodium nitrate 1g / L, ammonium chloride 1g / L, pH 6.2.

[0071] The steps of producing optically pure L-calcium lactate by the open in-situ product separation and fermentation method of the present invention are as follows:

[0072] (1) Slant culture: inoculate Bacillus coagulans H-1 on the slant medium, and culture at 50-60°C for 24-48 hours.

[0073] (2) Seed culture: inoculate the slant culture of step (1) into 30 mL seed medium under sterile conditions, add 40 g / L calcium carbonate to control the pH of the fermentation broth, and culture at 50-60 °C for 10 ~ 24 hours...

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Abstract

The invention discloses bacillus coagulans H-1 and a method using the bacillus coagulans H-1 for calcium lactate production by in-situ product separating and fermentation. The strain is Bacillus coagulans H-1, and the accession number is CCTCC (China Center For Type Culture Collection) NO:M2013105. Calcium lactate with a high optical purity is obtained by seed solution culture and then 50 to 250 hours of fermentation at 50-60 DEG C. Crystallized calcium lactate crystals can be obtained by continuous fermentation with an in-situ product separating and fermentation technology until the final production concentration in a fermentation tank is 167-171g / L, the production rate can be up to 5g / L / h, the optical purity can be up to 99.7%, and the conversion rate can be up to 0.95g / g. Semi continuous fermentation process can be repeatedly combined with the in-situ product separating and fermentation technology according to needs, the separation process is simple, economic, efficient, environmental-friendly, and sustainable. According to the method for calcium lactate production by in-situ product separating and fermentation, the cost is saved, meanwhile the production efficiency is improved, and the method has important industrial application value.

Description

technical field [0001] The invention belongs to the production method of calcium lactate in the technical field of biological fermentation engineering, and in particular relates to a strain of bacillus coagulans and a method for continuously producing optically pure calcium lactate by using the bacteria to carry out open-type in-situ product separation and fermentation. Background technique [0002] Lactic acid, also known as α-hydroxypropionic acid, can be divided into L-lactic acid (left-handed), D-lactic acid (dextral) and racemic lactic acid according to different optical activities. Lactic acid is a multipurpose organic acid, which can be widely used in food, medicine, chemical industry, printing and dyeing and other fields. Among them, the most important and widest industrial application is as a monomer for the synthesis of polylactic acid. Polylactic acid has good biodegradability and biocompatibility. It is not only very promising to become a substitute for petroleu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/56C12R1/07
Inventor 许平徐轲倪俊
Owner SHANGHAI JIAO TONG UNIV
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