Method for detecting yield of royal jelly by using bee LOC726515 gene

Abstract

The invention relates to a method for detecting the yield of royal jelly by using a bee LOC726515 gene. The method comprises bee sample collection, bee head RNA (Ribose Nucleic Acid) extraction, bee head cDNA (complementary Deoxyribonucleic Acid) synthesis, primer design, Qrt-PCR (quantitative Real-Time Polymerase Chain Reaction) reaction system and conditions, and royal jelly yield performance identification. The invention provides a fluorescent quantitative PCR method which is capable of identifying good or bad royal jelly yield performance of a swarm of bees more scientifically and accurately at the molecular biology level, aiming at the problem that the production of the royal jelly is enormously affected by conditions such as the population of the swarm of bees, nectar sources and weather, and as a result, the study period of the royal jelly production traits can be greatly shortened, the honeybee breeding speed can be increased, the cost of studying the royal jelly production traits and the cost of breeding can be reduced, and the working strength of the field study of the royal jelly yield performance also can be reduced.

Description

technical field [0001] The invention relates to a method for identifying the yield performance of royal jelly by using fluorescent quantitative PCR technology, in particular to a method for detecting the yield of royal jelly by using the honeybee LOC726515 gene. Background technique [0002] my country is a big beekeeping country, and it is also the main producer of royal jelly. More than 90% of the world's royal jelly is produced in China, and royal jelly-yielding bees are a unique bee resource in my country. For a long time, my country's bee industry scientific and technological workers have been committed to the related research on the genetic breeding of bees with high royal jelly yield, in order to find the genetic markers related to the high-yield traits of royal jelly, and serve the high-yield breeding of bee royal jelly. The genetic markers related to the high yield of royal jelly were studied at the level of morphology, cytology, biochemistry and molecular level. A...

AI Technical Summary

Problems solved by technology

They believed that the number of bee species and sample size in the above experiment was too small, and the sample selection was not very scientific. It cannot be simply inferred that W316bp can be used as a marker of high-yielding royal jelly just because three bees with high-yielding royal jelly have it but not among the low-yielding bees. Potentially related to other biological traits of four different bee species

Images