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A Rapid Identification Method for Grape Phylloxera Resistance

A technology of grape phylloxera and identification method, which is applied in the field of rapid detection of the resistance of the destructive pest grape phylloxera, and can solve the problems of long time-consuming and affecting the accuracy of identification results, etc.

Inactive Publication Date: 2015-12-09
NORTHWEST A & F UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the resistance identification needs to be carried out under special isolation conditions to avoid the spread of pests. In addition, the identification requires more than 2 years of repeated test identification, which takes a long time, and the environmental conditions during the operation may affect the accuracy of the identification results.

Method used

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  • A Rapid Identification Method for Grape Phylloxera Resistance
  • A Rapid Identification Method for Grape Phylloxera Resistance

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Embodiment Construction

[0011] The present invention will be described in detail below in conjunction with the accompanying drawings.

[0012] The invention provides a method for judging whether the grapevine phylloxera resistance exists by detecting the DNA molecular marker closely linked with the grapevine phylloxera resistance trait gene. The present invention is based on the microsatellite (Simple sequence repeat, SSR) molecular marker technology, with the oligodeoxynucleotide sequences Seq01 and Seq02 provided by the present invention as the SSR primer pair, with the DNA of the grape rootstock to be detected as a template, for phylloxera The specific DNA fragment linked to the resistance, that is, the DNA molecular marker, was amplified by Polymerase chain reaction (PCR), and after the amplified product was detected by electrophoresis, a specific DNA band (variety) with a size of about 169 bp was screened out. There is no difference in length between them), and there is a close linkage relations...

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Abstract

The invention provides a rapid identification method for phylloxera resistance. The method comprises the following steps: by taking grape stock to be detected as a DNA template, carrying out polymerase chain reaction amplification on the DNA molecular marker interlocked with the phylloxera resistance; and carrying out electrophoretic detection on the product obtained by amplification, if a DNA stripe corresponding to the DNA molecular marker exists in the electrophoretic result, showing that corresponding grape stock has phylloxera resistance, and if the DNA stripe corresponding to the DNA molecular marker does not exist in the electrophoretic result, showing that corresponding grape stock does not have phylloxera resistance. The method provided by the invention can rapidly differentiate whether grape stock has phylloxera resistance or not and solves the problem that existing phylloxera resistance identification requires a special isolating condition, is easily influenced by environmental conditions and takes a long time.

Description

technical field [0001] The invention belongs to the technical field of grape breeding and relates to the rapid detection of the resistance to the destructive pest phylloxera. Background technique [0002] Grape phylloxera is a destructive insect pest of grapes. It mainly harms grapes by feeding on the roots. It is difficult to prevent and control with conventional plant protection techniques. Currently the most effective preventive method is to use phylloxera-resistant rootstocks for grafting. However, in the process of grape rootstock breeding, whether the selected rootstock has phylloxera resistance needs to be identified for the rootstock’s resistance to phylloxera. Usually, the roots of grape rootstocks are artificially inoculated with phylloxera and the number of nodules formed by infection is counted for identification. , No nodules or a small number of nodules are resistant, and those with a large number of nodules are susceptible. However, the identification of res...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/156
Inventor 张军科张娟
Owner NORTHWEST A & F UNIV
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