DNA Molecular Marker for Rapid Identification of Grape Rootstock Phylloxera Resistance and Its Application

Abstract

The invention discloses a DNA (Deoxyribonucleic Acid) molecular marker for rapidly identifying phylloxera resistance of grape stock. The method comprises the following steps: by taking grape stock to be detected as a DNA template and oligodeoxynucleotide sequences Seq01 and Seq02 as a primer pair, carrying out polymerase chain reaction amplification on the DNA molecular marker interlocked with the phylloxera resistance; and carrying out electrophoretic detection on the product obtained by amplification, if a DNA stripe corresponding to the DNA molecular marker exists in the electrophoretic result, showing that corresponding grape stock has phylloxera resistance, and if the DNA stripe corresponding to the DNA molecular marker does not exist in the electrophoretic result, showing that corresponding grape stock does not have the phylloxera resistance. The molecular marker can be used for DNA molecular marker-assisted selective breeding for phylloxera resistance character. By taking a special DNA molecular marker stripe as a road sign, the phylloxera resistance gene can be precisely positioned or the phylloxera resistance gene is approached by a genome walking method, thereby laying a foundation for cloning the phylloxera resistance gene.

Description

Technical field: [0001] The invention belongs to the technical field of grape breeding in agricultural plant breeding, and relates to a DNA molecular marker used for rapid identification of resistance to phylloxera, a destructive pest of grapes, and the DNA molecular marker used as a DNA molecular marker for phylloxera-resistant breeding of grape rootstocks for early detection. Application of assisted selection in grape breeding. Background technique: [0002] Grape phylloxera is a destructive insect pest of grapes, which mainly harms grapes by feeding on young roots in the soil. Conventional plant protection techniques are difficult to prevent and control. At present, the most effective preventive method is to use phylloxera-resistant rootstocks for grafting. However, in the process of grape rootstock breeding, whether the selected rootstock has phylloxera resistance needs to be identified for the rootstock’s resistance to phylloxera. Usually, the roots of grape rootstocks...

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Problems solved by technology

However, the resistance identification needs to be carried out under special isolation conditions to avoid the spread of pests. In addition, the identification requires more than 2 years of repeated test identification, which takes a long time, and the environmental conditions during the operation may affect the accuracy of the identification results.

The patent of the invention provides a DNA molecular marker for grape phylloxera resistance, which can quickly distinguish whether the grape rootstock to be tested has grape phylloxera resistance, thereby solving the need for specific isolation conditions and being easily affected by environmental conditions in the identification of grape phylloxera resistance and require a longer period of time

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