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Long non-coding RNA (ribonucleic acid) related with endometrial cancer, as well as siRNA (short interfering ribonucleic acid) and application thereof

An endometrial cancer, non-coding technology, applied to long non-coding RNA and its siRNA and application fields, to achieve the effect of decreasing proliferation activity

Inactive Publication Date: 2014-07-23
THE FIRST AFFILIATED HOSPITAL OF MEDICAL COLLEGE OF XIAN JIAOTONG UNIV
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Problems solved by technology

[0006] However, functional lncRNA or lncRNA molecular markers in endometrial cancer are rarely reported.

Method used

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  • Long non-coding RNA (ribonucleic acid) related with endometrial cancer, as well as siRNA (short interfering ribonucleic acid) and application thereof
  • Long non-coding RNA (ribonucleic acid) related with endometrial cancer, as well as siRNA (short interfering ribonucleic acid) and application thereof
  • Long non-coding RNA (ribonucleic acid) related with endometrial cancer, as well as siRNA (short interfering ribonucleic acid) and application thereof

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Embodiment Construction

[0033] The present invention will be further described in detail below in conjunction with specific embodiments, which are for explanation rather than limitation of the present invention.

[0034] The present invention obtains information about long non-coding RNA molecules that are highly expressed in endometrial cancer tissues by analyzing and screening the differences in the expression levels of long non-coding RNA molecules in endometrial cancer and adjacent tissues. According to the partial sequence of the molecule published in UCSC, the nested PCR primers for 5'RACE and 3'RACE were designed to obtain the sequence information of the 5'and 3'ends of the long non-coding RNA molecule. And after the overlapping regions of 5'RACE and 3'RACE sequences are spliced, the full-length cDNA sequence of the long non-coding RNA molecule is obtained. Primers were designed based on the full-length cDNA sequence and a full-length truncated sequence was obtained. And design and screen out ef...

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Abstract

The invention discloses long non-coding RNA (ribonucleic acid) related with endometrial cancer, as well as siRNA (short interfering ribonucleic acid) and application thereof. The total length of cDNA (complementary deoxyribonucleic acid) of a gene is 1867bp, and the sequence is as shown in SEQ. ID. NO. 3. After the specific sequence is obtained, the sequence of the siRNA is designed for transfection; after a fragment is adopted for knocking down the expression of molecules of the long non-coding RNA in HEC-1-B cells, the proliferative activity of tumor cells is found to be lowered. Thus, the function of the gene can be related with the proliferation capability of cancer cells. After the interfering fragment siRNA1535 is adopted for knocking down the expression level of the gene, the proliferative activity of the tumor cells is obviously reduced, and the long non-coding RNA can be further applied to preparation of anti-tumor medicaments.

Description

Technical field [0001] The invention belongs to the technical field of tumor molecular biology, and relates to a long non-coding RNA related to endometrial cancer and its siRNA and applications. Background technique [0002] Endometrial cancer (endometrial eareinoma, EC) is one of the three common malignant tumors of female reproductive organs, accounting for 20-30% of female reproductive tract malignant tumors, and is one of the most common reproductive tract malignant tumors worldwide. The morbidity and fatality rates have an upward trend. In some European and American countries, endometrial cancer has become the first gynecological malignant tumor. The incidence of endometrial cancer in China is also increasing. The increase in the incidence of endometrial cancer may be related to the prolonged life expectancy, the increase in obesity, and the application of exogenous estrogen. [0003] By comparing genomic and proteomic studies, scientists have found that there are a large n...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/10A61K48/00A61P35/00
Inventor 陈葳李旭翟文薛梅吴文婧
Owner THE FIRST AFFILIATED HOSPITAL OF MEDICAL COLLEGE OF XIAN JIAOTONG UNIV
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