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Method for extracting deoxynojirimycin from white mulberry and purifying deoxynojirimycin

A technology of deoxynojirimycin and purification method, which is applied in the field of extraction and purification of deoxynojirimycin in mulberry trees, and can solve the problems that the extraction rate and separation effect need to be improved.

Active Publication Date: 2014-07-23
湖州柳荫生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction rate and separation effect of this method still need to be improved.

Method used

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  • Method for extracting deoxynojirimycin from white mulberry and purifying deoxynojirimycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Water extraction: Take 100kg of mulberry leaves and dry them in the sun, then add 800kg of deionized water, and place them in a tank-type countercurrent extraction equipment for three-cycle extraction including the first cycle, the second cycle, and the third cycle, and the irrigation group For each group of three extraction tanks used in series, the countercurrent extraction flow rate is 25min / tank, and the extraction temperature is 80°C;

[0033] The first cycle is countercurrent extraction from the first extraction tank: specifically, the dried mulberry leaves and 800kg deionized water are added in equal parts to the first extraction tank, the second extraction tank and the third extraction tank; the first extraction The medicinal liquid extracted from the tank for the first time is filtered and put into the liquid storage tank for subsequent use, and 400kg of distilled water is added to the second extraction of the first extraction tank and the third extraction of th...

Embodiment 2

[0042] Water extraction: Take 100kg of mulberry leaves and dry them in the sun, then add 1200kg of deionized water with the weight of the dried mulberry leaves, and place them in a tank-type countercurrent extraction equipment, including at least the first cycle, the second cycle and the third cycle. For the three-cycle extraction of the cycle, the irrigation group is used in series with three extraction tanks in each group, the countercurrent extraction flow rate is 35min / tank, and the extraction temperature is 110°C;

[0043] The first cycle is to start countercurrent extraction from the first extraction tank: specifically, add the dried mulberry leaves and 1200kg deionized water into the first extraction tank, the second extraction tank and the third extraction tank in equal parts; the first extraction The medicinal liquid extracted from the tank for the first time is filtered and put into the liquid storage tank for subsequent use, and 500kg of distilled water is added to t...

Embodiment 3

[0052] Water extraction: Take 100kg of mulberry leaves and dry them in the sun, then add 1000kg of deionized water with the weight of the dried mulberry leaves, and place them in a tank-type countercurrent extraction equipment, including at least the first cycle, the second cycle and the third cycle. For the three-cycle extraction of the cycle, the irrigation group is used in series with three extraction tanks in each group, the countercurrent extraction flow rate is 30min / tank, and the extraction temperature is 100°C;

[0053] The first cycle is to start countercurrent extraction from the first extraction tank: specifically, add the dried mulberry leaves and 1000kg deionized water into the first extraction tank, the second extraction tank and the third extraction tank in equal parts; the first extraction The medicinal liquid extracted from the tank for the first time is filtered and put into the liquid storage tank for standby, and the second extraction of the first extraction...

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Abstract

The invention relates to a method for extracting ingredients of white mulberry and in particular relates to a method for extracting deoxynojirimycin from the white mulberry and purifying deoxynojirimycin. The method for extracting deoxynojirimycin from the white mulberry and purifying deoxynojirimycin comprises the following steps: carrying out water extraction, namely airing leaves of the white mulberry, then adding deionized water, boiling at constant temperature and extracting, filtering and collecting filtrate, adding the deionized water into filter residue and then extracting again, putting filtrate together, adding trichloroacetic acid and centrifuging, so that first supernate is obtained; loading onto a column, namely purifying the first supernate by adopting a reversed phase chromatography column, so that liquid containing deoxynojirimycin is obtained; carrying out ethanol precipitation and taking supernate, namely firstly adding ethanol with alcohol content of 55-65% into the liquid containing deoxynojirimycin obtained after the step of loading onto the column is finished, stirring for 3-8 minutes, then adding ethanol with alcohol content of 70-80%, and stirring for 3-5 minutes, so that second supernate is obtained; concentrating, namely concentrating the second supernate, so that concentrated liquor is obtained; and carrying out microwave vacuum drying, namely carrying out microwave vacuum drying on the concentrated liquor, so that a powder product containing deoxynojirimycin is obtained. The method for extracting deoxynojirimycin from the white mulberry and purifying deoxynojirimycin has the advantages of high extraction and purification efficiency and good separating effect.

Description

technical field [0001] The invention relates to a method for extracting mulberry components, in particular to a method for extracting and purifying deoxynojirimycin in mulberry. Background technique [0002] As a dual-purpose plant for medicine and food, mulberry leaves contain a variety of active ingredients, which have good effects on lowering blood sugar, blood fat, and weight loss. In recent years, scholars at home and abroad have conducted in-depth research on mulberry leaves, proving that polysaccharides, flavonoids and alkaloids in mulberry leaves have good hypoglycemic effects. Chen Fujun et al. reported that the total polysaccharides (TPM) of mulberry leaves extracted from mulberry leaves had a significant hypoglycemic effect on four-star diabetic mice; the alkaloids in mulberry leaves can significantly reduce blood sugar levels, and the hypoglycemic effect of DNJ is particularly obvious . 1-Deoxynosumycin (DNJ), the chemical name is 3,4,5-trihydroxy-2 hydroxymeth...

Claims

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Application Information

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IPC IPC(8): C07D211/46
CPCC07D211/46
Inventor 柳秦桥陆巍杰
Owner 湖州柳荫生物科技有限公司
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