Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Molecular internal standard substance for identifying authenticity of objects and application of molecular internal standard substance

A technology for internal standards and articles, which is applied in the field of molecular internal standards for identifying authenticity of articles, can solve problems such as troublesome design of amplification primers and false positive results, and achieve the effect of improving specificity

Inactive Publication Date: 2014-07-02
OCEAN UNIV OF CHINA
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a molecular internal standard for identifying the authenticity of articles and its application, aiming to solve the problem of traditional PCR amplification technology being interfered by nucleic acid molecules contained in food, false positive results, and amplification problems. Adding troublesome problems in primer design

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular internal standard substance for identifying authenticity of objects and application of molecular internal standard substance
  • Molecular internal standard substance for identifying authenticity of objects and application of molecular internal standard substance
  • Molecular internal standard substance for identifying authenticity of objects and application of molecular internal standard substance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0036] Two nucleotide sequences B1 and B-1 are designed as molecular internal standards, wherein the sequences B1 and B-1 are as follows:

[0037] B1:

[0038]

[0039] B-1:

[0040]

[0041] The underlined part of the sequence B1 and B-1 is a palindromic sequence that can form a hairpin structure. For example, the structure of the sequence B1 is as follows;

[0042] TATAGC-5'

[0043]

[0044] The wavy sequence parts of the sequences B1 and B-1 cannot form a hairpin structure, which contains an endonuclease cutting site (AATT), and the wavy sequence parts of B1 and B-1 can be complementary paired.

[0045] Sequences B1 and B-1 contain two restriction endonuclease cutting sites BstUI (5'-CGCG-3') and Tsp509I (5'-AATT-3'), and the amplified products can be digested by these two Digested by endonucleases.

[0046] The endonuclease used in the embodiment of the present invention is Vent (exo-) DNA polymerase, which is a thermophilic polymerase and has strand displaceme...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a molecular internal standard substance for identifying the authenticity of objects and an application of the molecular internal standard substance. Two nucleotide sequences are designed so as to serve as the molecular internal standard substance which is directly added to liquid food or other goods; a specific amplification and detection method is used for detecting whether the internal standard substance exists so as to judge the authenticity of the liquid food or goods. The molecular internal standard substance has the advantages that nucleic acid molecules with certain sequences are designed so as to serve as the internal standard substance for identifying the authenticity of the goods, primers do not need to be additionally added in a detection identifying process, the trouble caused by using other methods for designing and amplifying the primers is avoided, the molecular internal standard substance is free of interference of nucleic acid molecules contained in food and has strong accuracy and strong specificity, particularly the reaction condition is constant temperature, and the complicated operations such as temperature control are reduced.

Description

technical field [0001] The invention belongs to the technical field of anti-counterfeiting identification, and in particular relates to a molecular internal standard used for identifying the authenticity of articles and its application. Background technique [0002] For the current food, especially the expensive liquor and health products, the current anti-counterfeiting measures mainly start with the outer packaging. Outer packaging anti-counterfeiting technology mainly includes two categories: logo anti-counterfeiting and packaging structure anti-counterfeiting: logo anti-counterfeiting mainly uses high-tech technology to attach various logos to the outer packaging for easy identification by consumers; packaging structure anti-counterfeiting starts with the structure of the packaging, common There is a one-time break package. However, the outer packaging is easy to be counterfeited, and the markings on the outer packaging have disadvantages such as being easily scratched,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/166
Inventor 梁兴国王鹏飞
Owner OCEAN UNIV OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products