Three-dimensional paper-based microfluidics and manufacture method thereof

Abstract

The invention provides three-dimensional paper-based microfluidics and a manufacture method thereof. The three-dimensional paper-based microfluidics is formed by overlapping and bookbinding of two-dimensional paper-based microfluidics. The manufacture method comprises the steps: designing a required chip pattern by drawing software, then printing wax onto the surface of a paper material with a printer, putting the printed paper material into a high-temperature container, baking, then taking out, and cooling to obtain the two-dimensional paper-based microfluidics; and then overlapping the two-dimensional paper-based microfluidics according to a designed way, next bookbinding the overlapped two-dimensional paper-based microfluidics together by a bookbinding device, and thus obtaining the three-dimensional paper-based microfluidics. The manufacture method has the advantages of simple operation, low cost and speediness, and has commercialization prospects.

Description

technical field [0001] The invention relates to the development of biochemical analysis products, and in particular provides a three-dimensional paper microfluidic chip and a manufacturing method thereof. Background technique [0002] Paper-based Microfluidics, referred to as paper chip, is a microfluidic chip that uses paper materials (such as filter paper, chromatographic paper, nitrocellulose membrane, etc.) as the chip production platform and biochemical analysis platform. In the paper chip system, the hydrophobic material divides the hydrophilic paper material into the area between the hydrophilic and hydrophobic phases to form a paper channel, and integrates basic operation units such as sampling, separation, reaction, and detection, and runs through the whole with controllable fluid. The system realizes the analysis function of the routine laboratory. In early 2007, the Whitesides group at Harvard University made paper chips for the first time and proposed this conce...

AI Technical Summary

Problems solved by technology

However, the double-sided adhesive or spray glue used in the above two methods will reduce the flow rate of the fluid in the hydrophilic channel in the paper chip on the one hand, and increase the time it takes for the fluid to penetrate the entire three-dimensional paper chip. On-site detection of test results is extremely unfavorable; on the other hand, double-sided adhesive or spray glue will be dissolved by organic solvents such as methanol, ethanol, ethyl acetate, etc., if the pretreatment reagent or detection reagent of the chip contains organic solvent, It will dissolve the adhesive between the layers of the chip, which will damage the paper chip and make the analysis process impossible

In addition, the detection reagent or pretreatment reagent circulating in the channel of the chip directly contacts the adhesive, which may also cause contamination and make the detection result inaccurate

Folding and pressing method, the whole chip is made on a piece of paper, and then completed by folding and pressing. The layers of paper chips are kept in contact with each other. This auxiliary clip is generally made of metal or other rigid plastics. The production process is cumbersome and the cost is high. It should also be different according to the different design structures of the chips.

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