Establishment method and application of isap fingerprint of Verbena flat tachycarpa

Abstract

The invention discloses a hemarthria compressa ISAP (Intron Sequence Amplified Polymorphism) fingerprint spectrum establishing method and applications of the method. According to the method, different hemarthria compressa germplasm DNA (Desoxvribose Nucleic Acid) fingerprint spectra are established by virtue of an ISAP molecular marking technology, which is conductive to rapid and accurate identification of clonal hemarthria compressa; compared with the conventional morphology testing and biochemical testing, the method is free from effects of growing environment and seasons, more reliable in result, time-saving and labor-saving; the method has the characteristics of being simple to operate, achieving visual practicability and the like, and can promote the wide application of the ISAP molecular marking technology in accurate, rapid and simple variety identification; besides, the method can be used for identifying the genetic variation on the clonal germplasm of hemarthria compressa, thereby meeting the requirements on early screening of variation on the clonal germplasm of hemarthria compressa and breeding of new varieties.

Description

technical field [0001] The invention relates to a method for establishing an ISAP fingerprint of Verbena flat tachycarpa and its application. Background technique [0002] Verbena flat spikes (Hemarthria compressa L.) is a perennial warm-season high-quality forage grass of the Poaceae Verbena genus. It has many stems and leaves, tender grass, and rich nutrition. Part of the water-soluble cellulose such as cellulose is slightly sweet in the mouth, which can be used for grazing, and can also be used to prepare hay and silage. Livestock like to eat and mainly rely on stems on the ground to reproduce. It has the advantages of wide adaptability, high quality and high yield, resistance to cutting, good palatability, high resistance and strong competition. important application value. [0003] With the rapid development of molecular biology technology, especially the establishment and maturity of molecular marker and gene marker technology, it provides an effective means for the ...

AI Technical Summary

Problems solved by technology

At present, there is no research on the DNA fingerprinting of Verbena spp. clones by ISAP marker technology, and there is no establishment of a perfect method for constructing the DNA fingerprinting of S.

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