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Compound aquatic microcapsule initial baits, preparation method and application of initial baits

An open bait, compound technology, applied in the application, animal feed, animal feed and other directions, can solve the problems of inability to use aquatic seedlings, low content of antibacterial active peptides, poor water solubility of fly maggot protein, etc., to maintain normal physiological environment and ecology. Balanced, nutritionally balanced, and the effect of improving disease resistance

Active Publication Date: 2014-06-18
JINLING INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the water solubility of fly maggot protein is poor, and the dissolution rate is low, so it cannot be effectively used for aquatic seedlings.
In addition, the content of antibacterial active peptides in fly maggot protein powder is also very low, which limits its further utilization

Method used

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  • Compound aquatic microcapsule initial baits, preparation method and application of initial baits
  • Compound aquatic microcapsule initial baits, preparation method and application of initial baits
  • Compound aquatic microcapsule initial baits, preparation method and application of initial baits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1.1 Preparation of fly maggot protein polypeptide liquid:

[0041] Rinse 1kg of housefly maggots hatched from pig, cow or chicken manure with clean water, add 500mL of 0.05M phosphate buffer, mix well with a high-speed homogenizer, centrifuge at 6000rpm for 20 minutes, and take the supernatant for storage , add the same volume of 0.05M phosphate buffer to the centrifugal pellet and mix well, adjust the pH to 3 with phosphoric acid, add acid protease and incubate at 37°C for 1 to 1 hour, then adjust the pH to 8 with NaOH, and also incubate at 37°C for 1 hour, the enzymatic hydrolysis product and Mix the aforementioned centrifuged supernatants, add 0.05M phosphate buffer to adjust the protein concentration to not less than 10mg / mL, and the content of amino nitrogen to not less than 10mg / 100mL to obtain the fly maggot protein polypeptide solution, which is tested against Escherichia coli and Salmonella The antibacterial activity is less than 1mg / mL. 1.2 Preparation of edi...

Embodiment 2

[0052] 2.1 Preparation of fly maggot protein polypeptide liquid:

[0053] Rinse 10kg of housefly maggots hatched from pig, cow or chicken manure with clean water, add 5000mL of 0.05M phosphate buffer, and homogenize twice under the condition of 20-40MPa with a homogenizer to obtain a uniform emulsion; Centrifuge at 6000rpm for 20 minutes, take the supernatant and save it, add the same volume of 0.05M phosphate buffer to the centrifugal pellet and mix well, adjust the pH to 3 with phosphoric acid, add acid protease and incubate at 37°C for 3 hours, then adjust the pH to 8 with NaOH. Incubate at 37°C for 3 hours, mix the enzymatic hydrolysis product with the centrifuged supernatant, add 0.05M phosphate buffer to adjust the protein concentration to not less than 10mg / mL, and the amino nitrogen content to not less than 10mg / 100mL to obtain fly maggot protein Peptide solution.

[0054] 2.2 Preparation of Edible Fungi Fermentation Homogenate:

[0055] Take brewer's grains 20wt%, w...

Embodiment 3

[0066] Get the ratio of the bait prepared by 1 and 2 in the above-mentioned embodiment and mix evenly by the ratio of 15 kg / ton of natural feed, then feed the animals and feed the bait (the control group is conventional bait: spirulina, yeast, carmelium, egg yolk, etc.; the experimental group : 25% for conventional bait, 75% for the microcapsule bait of the present invention), the original operating procedure remains unchanged, and it is still three times a day: early (5:00), late (15:00), night (zero o’clock) or Twice in the morning and evening, it can also be used according to the local feeding method.

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Abstract

The invention discloses a compound aquatic microcapsule initial baits, a preparation and application of the initial baits. The initial baits are prepared by mixing the following main materials mixed in different proportion: maggot protein polypeptide, edible fungus fermentation liquid, lactobacillus, spirulina powder, soya lecithin and vegetable oil with sodium alga acid according to proportion, carrying out high-speed homogenizing, pressurizing, and spraying the mixture into CaCl2 liquid form micro capsules, and filtrating. The diameters of the initial baits are from micrometers to millimeters and the initial baits can suspend in water in a certain time. The initial baits are suitable for newly hatched aquatic seedlings in different growth stages, can strengthen body immunity, regulate ecological balance of intestinal and promote the growth and development. The initial baits meet the requirements on Green feed and feed addictives due to no antibiotics and shell element and no chemical residue, also have the advantages of low cost, convenience in usage and can be widely adopted in aquatic breeding and aquaculture fields.

Description

technical field [0001] The invention relates to a microcapsule bait for aquaculture, in particular to a microcapsule opening bait for aquatic seedlings and a preparation method and application thereof. Background technique [0002] Since hatched aquatic animals are often passive for the first time to open their mouths, the mortality rate of their seedlings is very high. The reason is that the natural environment cannot provide enough high-quality open feed, and the aquaculture water body has accumulated a large number of pathogenic microorganisms due to the perennial cycle of aquaculture, which is not conducive to the growth and development of aquatic seedlings. Therefore breeding seedlings in large quantities is the key of raising aquatic animals artificially, and the development of opening bait then becomes the technical key. The basic requirement for open bait for aquatic seedlings is to have balanced nutrients composed of high animal protein, so that the juvenile fry th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/16A23K1/00
Inventor 陈涛茅慧华戴鼎震方光远程泽信蒋加进
Owner JINLING INST OF TECH
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